Comparison of Three DNA Extraction Methods for Detection Echinococcus granulosus Isolated from Sheep and Cows

  • Dunya N. Ahmed Dept. of Nursing, Medical Technology, Institute of Baghdad/ University of Middle Technical.
  • Salwa S. Muhsin **Dept. of Community Health, Medical Technology, Institute of Baghdad/ University of Middle Technical
  • Abudulla L. Chyiad Dept. of Anesthesia, Medical Technology Institute of Baghdad/ University of Middle Technical
Keywords: DNA extraction methods, hydatid cysts. Echinococcus granulosus

Abstract

Background: Hydatosis caused by Echinococcus granulosus dog tap worm is zoonotic infection and economic importance and to public health constitutes a major threat in certain regions of the Middle East. There is an establishment of preventive and control strategy for characterization of E.granulosus in all endemic area which is essential in all molecular studies, to check the DNA of the parasite.
Objective: Our study aimed to obtain the best from three extractions DNA methods from hydatid cyst protoscolecses isolated from sheep in Al-shawlla abattoir in Baghdad.
Subjects and Methods: Three methods were used to extract DNA samples (boiling, crushing and commercial) DNA samples were performed with electrophoreses on 1.3% agarose. Regarding DNA for methods were compared by time and facility and cost-effectiveness.
Results: The most method was boiling and crushing DNA extraction because of their easy, quickness besides the commercial kit method which had good bands on gel electrophoresis but had most cost effectiveness and time.
Conclusion: The most method were boiling and crushing using for DNA extraction

 

 

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Author Biography

Dunya N. Ahmed, Dept. of Nursing, Medical Technology, Institute of Baghdad/ University of Middle Technical.




Published
2018-04-01
How to Cite
1.
Ahmed D, Muhsin S, Chyiad A. Comparison of Three DNA Extraction Methods for Detection Echinococcus granulosus Isolated from Sheep and Cows. J Fac Med Bagdad [Internet]. 1Apr.2018 [cited 24Jan.2020];60(1):62-4. Available from: http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/48