Association between Tissue Polypeptide Specific Antigen and Vascular Endothelial Growth Factor in Colorectal Cancer Patients and their Relation with P53 Expression and Global DNA Methylation

Dalya Falih; Rakad AL-Jumaily

doi:10.32007/jfacmedbagdad.2081

المؤلفون

Dalya Falih Scholar resercher https://orcid.org/0009-0005-8774-1761
Rakad AL-Jumaily Department of Biology, College of Science, University of Baghdad https://orcid.org/0000-0001-9455-6410

DOI:

https://doi.org/10.32007/jfacmedbagdad.2081

الكلمات المفتاحية:

Colorectal cancer, Tissue polypeptide antigen, Vascular endothelial growth factor, DNA methylation.

الملخص

سرطان القولون والمستقيم (CRC) هو مرض شديد الخطورة يتسبب في مشاكل طبية سريعة التطور وله معدل وفيات مرتفع. يمكن تصنيف مُسْتَضِدٌّ مُنَاوِعٌ للنَّسيج (TPS) كمؤشر حيوي في سرطان القولون والمستقيم وأنواع أخرى من السرطانات. لوحظ ان لعامل نمو بطانة الأوعية الدموية (VEGF) دور علاجي في تكوين أوعية دموية جديدة. قد تساهم مثيلة الحمض النووي بتقليل انتشار السرطان. كانت هذه الدراسة مصممة لقياس الدور المحتمل لمستويات TPS و VGEF في تقدم CRC بالإضافة إلى علاقتها بتعبير P53 و مثيلة الحمض النووي (5mC) وايضا الحالة البيولوجية لمرضى CRC مثل مرحلة المرض ودرجة تطور المرض وعمق غزو النسيج. شملت الدراسة الحالية 30 متطوعًا سليما كمجموعة سيطرة و 60 مريضًا مصابًا بـ CRC. تم فحص جميع المشاركين لمستويات TPS و VGEF في المصل باستخدام اختبار ELISA. أيضًا ، تم استخراج الحمض النووي والحمض النووي الريبي وتم تقييم مستويات تعبير (5mC) و P53. كانت هناك اختلافات كبيرة في مستويات المصل لكل من TPS و VGEF بين مرضى CRC والسيطرة. فيما يتعلق بالسمات الديموغرافية للمرضى كانت هناك فروق ذات دلالات إحصائية في مستويات المصل لكل منTPS ، VGEF. . لم تكشف الدراسة عن وجود ارتباط معنوي بين مستويات التعبير الجيني لجين ال P53 ، مثيلة الحمض النووي , وكذلك TPS و VGEF في مجاميع الدراسة. قد تلعب مستويات TPS و VGEF في مصل المرضى دورًا مهمًا في تطوير CRC. يعتبر مستوى VGEF مفيدًا في التمييز بين CRC ومجموعة السيطرة ، في حين أن مستوى TPS هو عامل تنبؤي لاستمرار مرض CRC.

التنزيلات

تنزيل البيانات ليس متاحًا بعد.

المراجع

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2018. CA: a cancer journal for clinicians. 2018 Jan;68(1):7-30. https://doi.org/10.3322/caac.21442.

Borgmästars E, Lundberg E, Öhlund D, Nyström H, Franklin O, Lundin C, Jonsson P, Sund M. Circulating Tissue Polypeptide-Specific Antigen in Pre-Diagnostic Pancreatic Cancer Samples. Cancers. 2021 Oct 23;13(21):5321. https://doi.org/10.3390/cancers13215321.

Jelski W, Mroczko B. Biochemical markers of colorectal cancer–present and future. Cancer management and research. 2020;12:4789. https://doi.org/10.2147/cmar.s253369.

Goulart A, Ferreira C, Rodrigues A, Coimbra B, Sousa N, Leão P. The correlation between serum vascular endothelial growth factor (VEGF) and tumor VEGF receptor 3 in colorectal cancer. Annals of Surgical Treatment and Research. 2019 Jul 1;97(1):15-20. https://doi.org/10.4174/astr.2019.97.1.15.

Hussein SM, Hassani HH, Risan MH. Mutations in Exon 4 of ESR1 Gene in Iraqi Women with Breast Cancer.

Orjuela S, Menigatti M, Schraml P, Kambakamba P, Robinson MD, Marra G. The DNA hypermethylation phenotype of colorectal cancer liver metastases resembles that of the primary colorectal cancers. BMC cancer. 2020 Dec;20(1):1-3. https://doi.org/10.1186/s12885-020-06777-6.

Phipps AI, Limburg PJ, Baron JA, Burnett-Hartman AN, Weisenberger DJ, Laird PW, Sinicrope FA, Rosty C, Buchanan DD, Potter JD, Newcomb PA. Association between molecular subtypes of colorectal cancer and patient survival. Gastroenterology. 2015 Jan 1;148(1):77-87. https://doi.org/10.1053/j.gastro.2014.09.038.

Jung G, Hernández-Illán E, Moreira L, Balaguer F, Goel A. Epigenetics of colorectal cancer: biomarker and therapeutic potential. Nature reviews Gastroenterology & hepatology. 2020 Feb;17(2):111-30. https://doi.org/10.1038/s41575-019-0230-y.

Zehir A, Benayed R, Shah RH, Syed A, Middha S, Kim HR, Srinivasan P, Gao J, Chakravarty D, Devlin SM, Hellmann MD. Mutational landscape of metastatic cancer revealed from prospective clinical sequencing of 10,000 patients. Nature medicine. 2017 Jun;23(6):703-13.

Xiao Q, Werner J, Venkatachalam N, Boonekamp KE, Ebert MP, Zhan T. Cross-Talk between p53 and Wnt Signaling in Cancer. Biomolecules. 2022 Mar 15;12(3):453. https://doi.org/10.3390/biom12030453.

Łukaszewicz-Zając M, Mroczko B. Circulating biomarkers of colorectal cancer (CRC)—their utility in diagnosis and prognosis. J of Clin. Med. 2021 May 28; 10(11): 2391. https://doi.org/10.3390/jcm10112391.

Li XJ, Ren ZJ, Tang JH. MicroRNA-34a: a potential therapeutic target in human cancer. Cell death & disease. 2014 Jul;5(7):e1327-. https://doi.org/10.1038/cddis.2014.270.

Ohtani S, Kagawa S, Tango Y, Umeoka T, Tokunaga N, Tsunemitsu Y, Roth JA, Taya Y, Tanaka N, Fujiwara T. Quantitative analysis of p53-targeted gene expression and visualization of p53 transcriptional activity following intratumoral administration of adenoviral p53 in vivo. Molecular cancer therapeutics. 2004 Jan;3(1):93-100. https://doi.org/10.1158/1535-7163.93.3.1.

Wang W, Lv YF, Zhang YJ, Dong WJ, Zhang Y. Generation of a human induced pluripotent stem cell line PUMCi001-A from a patient with Krabbe disease. Stem cell research. 2020 Oct 1;48:101937. https://doi.org/10.1016/j.scr.2020.101937.

Siegel RL, Jakubowski CD, Fedewa SA, Davis A, Azad NS. Colorectal cancer in the young: epidemiology, prevention, management. American Society of Clinical Oncology Educational Book. 2020 Apr 21;40:e75-88. https://doi.org/10.1200/edbk_279901.

Kneuertz PJ, Chang GJ, Hu CY, Rodriguez-Bigas MA, Eng C, Vilar E, Skibber JM, Feig BW, Cormier JN, You YN. Overtreatment of young adults with colon cancer: more intense treatments with unmatched survival gains. JAMA surgery. 2015 May 1;150(5):402-9. https://doi.org/10.1001/jamasurg.2014.3572.

Wang G, Wang Y, Yang X, Zhang Y, Lu Y, Li Y. The expression and diagnostic value of serum levels of EphA2 and VEGF-A in patients with colorectal cancer. Cancer Biomarkers. 2021 Jan 1;31(4):399-408. https://doi.org/10.3233/cbm-201745.

Zhang M, Wang Y, Wang Y, Jiang L, Li X, Gao H, Wei M, Zhao L. Integrative analysis of DNA methylation and gene expression to determine specific diagnostic biomarkers and prognostic biomarkers of breast cancer. Frontiers in Cell and Developmental Biology. 2020 Dec 7;8:529386. https://doi.org/10.3389/fcell.2020.529386.

Al-Rubaye RH, Al-Jumaily RM. Evaluation of oxidative stress activity and the levels of homocysteine, vitamin B12, and DNA methylation among women with breast cancer. Journal of Advanced Biotechnology and Experimental Theraputic. 2023 Jan; 6(1): 149-160. https://doi.org/10.5455/jabet.2023.d114.

Xiang Y, Huang Y, Sun H, Pan Y, Wu M, Zhang J. Deregulation of miR-520d-3p promotes hepatocellular carcinoma development via lncRNA MIAT regulation and EPHA2 signaling activation. Biomedicine & Pharmacotherapy. 2019 Jan 1;109:1630-9. https://doi.org/10.1016/j.biopha.2018.11.014.

Gorenjak V, Vance DR, Dade S, Stathopoulou MG, Doherty L, Xie T, Murray H, Masson C, Lamont J, Fitzgerald P, Visvikis-Siest S. Epigenome-wide association study in healthy individuals identifies significant associations with DNA methylation and PBMC extract VEGF-A concentration. Clinical Epigenetics. 2020 Dec;12(1):1-1. https://doi.org/10.1186/s13148-020-00874-w.

Zhang J, Wei Q, Dong D, Ren L. The role of TPS, CA125, CA15-3 and CEA in prediction of distant metastasis of breast cancer. Clinica Chimica Acta. 2021 Dec 1;523:19-25. https://doi.org/10.1016/j.cca.2021.08.027.

Xiao Q, Werner J, Venkatachalam N, Boonekamp KE, Ebert MP, Zhan T. Cross-Talk between p53 and Wnt Signaling in Cancer. Biomolecules. 2022 Mar 15;12(3):453. https://doi.org/10.3390/biom12030453.

Oh HH, Joo YE. Novel biomarkers for the diagnosis and prognosis of colorectal cancer. Intestinal research. 2020 Apr;18(2):168. https://doi.org/10.5217/ir.2019.00080.

Ali CA, Lafta FM, Al Sayyid MM, Al-Rekabi AA. BRCA1 Gene Expression is Down Regulated in Both Familial and Sporadic Breast Cancer Cases in Baghdad-Iraq. Iraqi Journal of Science. 2020 Jan 26:34-41. https://doi.org/10.24996/ijs.2020.61.1.4.

Berretta M, Alessandrini L, De Divitiis C, Nasti G, Lleshi A, Di Francia R, Facchini G, Cavaliere C, Buonerba C, Canzonieri V. Serum and tissue markers in colorectal cancer: State of art. Critical Reviews in Oncology/Hematology. 2017 Mar 1;111:103-16.

https://doi.org/10.1016/j.critrevonc.2017.01.007.

Ibraheem NI, Ali RH, Ismail MB. Kidney functions and electrolyte disturbance among Iraqi patients with bladder cancer. Journal of the Faculty of Medicine Baghdad. 2022;64(4):214-7. https://doi.org/10.32007/jfacmedbagdad.6441985.

Qasim TH, Ibraheem MJ, Jabarah MA. Impact of sociodemographic characteristics and drug-related side effects on quality of life of patients with hepatocellular carcinoma receiving sorafenib treatment in Iraqi hospitals. Journal of the Faculty of Medicine Baghdad. 2022;64(4):286-91. https://doi.org/10.32007/jfacmedbagdad.6442009.