Changes in levels of Interleukin-6 (IL-6) and Interleukin-8 (IL-8) in the Serum of Preterm Delivery Pregnant Women Affected by Gingivitis
DOI:
https://doi.org/10.32007/jfacmedbagdad.2152Keywords:
Gingivitis, Periodontal disease, preterm delivery, full term-delivery, Interleukine-6, Interleukin-8Abstract
Background: Periodontal disease increases local and systemic inflammatory responses in pregnant women, which may lead to premature delivery.
Objectives: To detect maternal serum levels of proinflammatory cytokine (Interleukine-6, Interleukin-8) with preterm delivery in pregnant women suffering from gingivitis.
Cases and methods: In this case-control study, a total of 85 pregnant women, were included in the study, of whom 55 had gingivitis: 25 preterm deliveries and 30 full-term deliveries. Thirty healthy pregnant women with healthy gingiva were in the control group. They were selected from Al-Ramadi Teaching Hospital for Gynecology and Pediatrics, from November 2022 to May 2023. Blood samples were collected from all participants and the biomarkers cytokines were detected by immunosorbent assay ( ) kits.
Results: The current study showed an increased level of interleukin-6 and interleukin-8 in the pre-term delivery gingivitis group followed by full-term-delivery pregnant groups having gingivitis compared with the control group, with statistically highly significant differences ( ).
Conclusion: Pregnant women with gingivitis with the highest levels of Interlukine-6 and Interlukine-8 were more prone to have premature delivery than those with lower levels of Interleukins and those without gingivitis.
Received: June, 2023
Accepted: Aug, 2023
Published: Jan..2024
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Abdulkareem AA, Al-Taweel FB, Al-Sharqi AJB, Gul SS, Sha A, Chapple ILC. Current concepts in the pathogenesis of periodontitis: from symbiosis to dysbiosis. J Oral Microbiol. 2023 Apr 2;15(1):2197779. https://doi.org/10.1080/20002297.2023.2197779
Al-Alousi HW. Frequency of Salivary Human Cytomegalovirus in Iraqi Patients with Chronic Periodontitis. Journal of the Faculty of Medicine Baghdad.2013; 55(2), 162-165.https://doi.org/10.32007/jfacmedbagdad.552647
Al-obaidi M, Al-Ghurabi BH. Association between Aggregatibacter actinomy-cetemcomitans bacterial load and NLRP3 inflam-masome activation in periodontitis patients with Diabetes. Journal of the Faculty of Medicine Baghdad, 2023;64(4), 268-275. https://doi.org/10.32007/jfacmedbagdad.6441972
Hameed NM, Alwaheb AM. Trajectories of Salivary Hormones in Pregnant Women with Anxiety and their Effect on Gingival Health Condition. Indian Journal of Forensic Medicine & Toxicology, 2022;16(1), 1458-1465.https://doi.org/10.37506/ijfmt.v16i1.17706
Ali SS, Ali MS, AL-Gebori AM. The influence and assessment of E-cadherin sera levels with periodontitis and gingivitis. Journal of Survey in Fisheries Sciences, 2023; 10(3S), 3151-3161.
Hill GB. Preterm birth: associations with genital and possibly oral microflora. Ann Periodontol. 1998 Jul;3(1):222-32. https://doi.org/10.1902/annals.1998.3.1.222
Spahr A, Klein E, Khuseyinova N, Boeckh C, Muche R, Kunze M, et al. Periodontal infections and coronary heart disease: role of periodontal bacteria and importance of total pathogen burden in the Coronary Event and Periodontal Disease (CORODONT) study. Arch Intern Med. 2006 Mar 13;166(5):554-9. .
https://doi.org/10.1001/archinte.166.5.554
Lieff S, Boggess KA, Murtha AP, Jared H, Madianos PN, Moss K, et al. The oral conditions and pregnancy study: periodontal status of a cohort of pregnant women. J Periodontol. 2004 Jan;75(1):116-26. https://doi.org/10.1902/jop.2004.75.1.116
Offenbacher S, Katz V, Fertik G, Collins J, Boyd D, Maynor G, et al. Periodontal infection as a possible risk factor for preterm low birth weight. J Periodontol. 1996 Oct;67(10 Suppl):1103-13. https://doi.org/10.1902/jop.1996.67.10s.1103
AL- Muhamadawy AK. Periodotnal health status and treatment needs among Iraqi dental students. Journal of the Faculty of Medicine Baghdad, 2010;51(4), 378-381.
Ertugrul AS, Sahin H, Dikilitas A, Alpaslan N, Bozoglan A. Comparison of CCL28, interleukin-8, interleukin-1β and tumor necrosis factor-alpha in subjects with gingivitis, chronic periodontitis and generalized aggressive periodontitis. J Periodontal Res. 2013 Feb;48(1):44-51. https://doi.org/10.1111/j.1600-0765.2012.01500.x
Daily ZA, Mohammed AN. Periodontal Health Status and Assessment of Osteocalcin levels in Saliva of Diabetic Patients and Systemically Healthy Persons (Comparative study). Journal of Baghdad College of Dentistry, 2017; 29(1), 89-95. https://doi.org/10.12816/0038632
Yokoyama M, Hinode D, Masuda K, Yoshioka M, Grenier D. Effect of female sex hormones on Campylobacter rectus and human gingival fibroblasts. Oral Microbiol Immunol. 2005 Aug;20(4):239-43. https://doi.org/10.1111/j.1399-302X.2005.00222.x
Paul AM, Victor DJ. Gana Prakash PS. Role of Interleukin-8 in periodontal disease. International Journal of Clinical Dental Science, 2012;3.
Lagdive SS, Marawar PP, Byakod G, Lagdive SB. Evaluation and comparison of interleukin-8 (IL-8) level in gingival crevicular fluid in health and severity of periodontal disease: a clinico-biochemical study. Indian J Dent Res. 2013 Mar-Apr;24(2):188-92. https://doi.org/10.4103/0970-9290.116675
Shahshahan Z, Hashemi L. Maternal serum cytokines in the prediction of preterm labor and response to tocolytic therapy in preterm labor women. Adv Biomed Res. 2014 May 28;3:126. https://doi.org/10.4103/2277-9175.133243
Dasanayake AP. Poor periodontal health of the pregnant woman as a risk factor for low birth weight. Ann Periodontol. 1998 Jul;3(1):206-12. https://doi.org/10.1902/annals.1998.3.1.206
Al Mosawi A O. Need and Demand According to Basic Periodontal Index to Patient with Periodontal Disease Attending Department of Periodontology at the College of Dentistry/University of Baghdad. Latin American Journal of Pharmacy, 2021;40(SI), 271-275.
Sanz M, Kornman K. Working group 3 of joint EFP/AAP workshop. Periodontitis and adverse pregnancy outcomes: consensus report of the Joint EFP/AAP Workshop on Periodontitis and Systemic Diseases. J Clin Periodontol. 2013 Apr;40 Suppl 14:S164-9. https://doi.org/10.1111/jcpe.12083
Stevens VL, Hoover E, Wang Y, Zanetti KA. Pre-Analytical Factors that Affect Metabolite Stability in Human Urine, Plasma, and Serum: A Review. Metabolites. 2019 Jul 25;9(8):156. https://doi.org/10.3390/metabo9080156
Hasegawa K, Furuichi Y, Shimotsu A, Nakamura M, Yoshinaga M, Kamitomo M, et al. Associations between systemic status, periodontal status, serum cytokine levels, and delivery outcomes in pregnant women with a diagnosis of threatened premature labor. J Periodontol. 2003 Dec;74(12):1764-70. https://doi.org/10.1902/jop.2003.74.12.1764
Keelan JA, Blumenstein M, Helliwell RJ, Sato TA, Marvin KW, Mitchell MD. Cytokines, prostaglandins, and parturition--a review. Placenta. 2003 Apr;24 Suppl A:S33-46. https://doi.org/10.1053/plac.2002.0948
von Minckwitz G, Grischke EM, Schwab S, Hettinger S, Loibl S, Aulmann M, et al. Predictive value of serum interleukin-6 and -8 levels in preterm labor or rupture of the membranes. Acta Obstet Gynecol Scand. 2000 Aug;79(8):667-72. PMID: 10949232. https://doi.org/10.1034/j.1600-0412.2000.079008667.x
Lewis DF, Barrilleaux PS, Wang Y, Adair CD, Baier J, Kruger T. Detection of interleukin-6 in maternal plasma predicts neonatal and infectious complications in preterm premature rupture of membranes. Am J Perinatol. 2001 Nov;18(7):387-91. https://doi.org/10.1055/s-2001-18694
Latorre Uriza C, Velosa-Porras J, Roa NS, Quiñones Lara SM, Silva J, Ruiz AJ, et al. Periodontal Disease, Inflammatory Cytokines, and PGE2 in Pregnant Patients at Risk of Preterm Delivery: A Pilot Study. Infect Dis Obstet Gynecol. 2018 Aug 1;2018:7027683. https://doi.org/10.1155/2018/7027683
Greig PC, Murtha AP, Jimmerson CJ, Herbert WN, Roitman-Johnson B, Allen J. Maternal serum interleukin-6 during pregnancy and during term and preterm labor. Obstet Gynecol. 1997 Sep;90(3):465-9. https://doi.org/10.1016/S0029-7844(97)00294-9
Dörtbudak O, Eberhardt R, Ulm M, Persson GR. Periodontitis, a marker of risk in pregnancy for preterm birth. J Clin Periodontol. 2005 Jan;32(1):45-52. https://doi.org/10.1111/j.1600-051X.2004.00630.x
Madianos PN, Bobetsis YA, Offenbacher S. Adverse pregnancy outcomes (APOs) and periodontal disease: pathogenic mechanisms. J Clin Periodontol. 2013 Apr;40 Suppl 14:S170-80. https://doi.org/10.1111/jcpe.12082
Hebisch G, Grauaug AA, Neumaier-Wagner PM, Stallmach T, Huch A, Huch R. The relationship between cervical dilatation, interleukin-6 and interleukin-8 during term labor. Acta Obstet Gynecol Scand. 2001 Sep;80(9):840-8.https://doi.org/10.1034/j.1600-0412.2001.080009840.x
Gücer F, Balkanli-Kaplan P, Yüksel M, Yüce MA, Türe M, Yardim T. Maternal serum tumor necrosis factor-alpha in patients with preterm labor. J Reprod Med. 2001 Mar;46(3):232-6. PMID: 11304864.
Van Dyke TE, van Winkelhoff AJ. Infection and inflammatory mechanisms. J Clin Periodontol. 2013 Apr;40 Suppl 14:S1-7. https://doi.org/10.1111/jcpe.12088
Carta G, Persia G, Falciglia K, Iovenitti P. Periodontal disease and poor obstetrical outcome. Clin Exp Obstet Gynecol. 2004;31(1):47-9. PMID: 14998188.
Goepfert AR, Jeffcoat MK, Andrews WW, Faye-Petersen O, Cliver SP, Goldenberg RL, et al. Periodontal disease and upper genital tract inflammation in early spontaneous preterm birth. Obstet Gynecol. 2004 Oct;104(4):777-83.
https://doi.org/10.1097/01.AOG.0000139836.47777.6d
Jayaprakash K, Khalaf H, Bengtsson T. Gingipains from Porphyromonas gingivalis play a significant role in induction and regulation of CXCL8 in THP-1 cells. BMC Microbiol. 2014 Jul 18;14:193. https://doi.org/10.1186/1471-2180-14-193
Pan W, Wang Q, Chen Q. The cytokine network involved in the host immune response to periodontitis. Int J Oral Sci. 2019 Nov 5;11(3):30. PMID: 31685798; PMCID:PMC6828663 . https://doi.org/10.1038/s41368-019-0064
Ide M, Papapanou PN. Epidemiology of association between maternal periodontal disease and adverse pregnancy outcomes--systematic review. J Clin Periodontol. 2013 Apr;40 Suppl 14:S181-94. PMID: 23627328. https://doi.org/10.1111/jcpe.12063
Mamalis A, Markopoulou C, Lagou A, Vrotsos I. Oestrogen regulates proliferation, osteoblastic differentiation, collagen synthesis and periostin gene expression in human periodontal ligament cells through oestrogen receptor beta. Arch Oral Biol. 2011 May;56(5):446-55. PMID: 21130420.https://doi.org/10.1016/j.archoralbio.2010.11.001
Nebel D, Jönsson D, Norderyd O, Bratthall G, Nilsson BO. Differential regulation of chemokine expression by estrogen in human periodontal ligament cells. J Periodontal Res. 2010 Dec;45(6):796-802. PMID: 20701669 .
https://doi.org/10.1111/j.1600-0765.2010.01308.x
Straub RH. The complex role of estrogens in inflammation. Endocr Rev. 2007 Aug;28(5):521-74.PMID: 17640948. https://doi.org/10.1210/er.2007-0001
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