Evaluation of the Active constituents, Antioxidant, and Antimicrobial Activities of Iraqi Euonymus Japonicus leaves using Ethyl Acetate Extract

Rasha A.  Khalaf; Thukaa z.  Abdul-Jalil

doi:10.32007/jfacmedbagdad.1991

Authors

Rasha A. Khalaf Department. of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Baghdad,, Baghdad, Iraq. https://orcid.org/0000-0003-4579-9955
Thukaa z. Abdul-Jalil Department. of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Baghdad, Baghdad, Iraq.

DOI:

https://doi.org/10.32007/jfacmedbagdad.1991

Keywords:

Antibacterial, , antioxidant and ethyl acetate fraction, , Euonymus japonicus,, polyphenolic compounds.

Abstract

Background: Euonymus japonicus is one species of celastraceous family used as decorative plant and traditional Chinese medicine. The lack of information about the main active constituents and the possible biological activities of Iraqi Euonymus japonicus leaves considered as a motivation to start this in vitro study

Objectives: to identify the phytochemical components and to evaluation antioxidant and antimicrobial activities

Material and Methods: The chemical composition of Iraqi Euonymus japonicus leaves was identified and analyzed using the Reversed-Phase High-Performance Liquid Chromatography approach and the antioxidant properties were measured by free radical - scavenging assay DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate). Furthermore, the antibacterial properties were evaluated via agar well diffusion method against two pathogenic bacteria Staphylococcus- aureus, E. coli and Candida albicans.

Results: The results showed that the main active constituents of Euonymus japonicus leaves in ethyl acetate fraction were Naringenin, vitexin, Kaempferol, Apigenin and quercetin respectively.in addition antioxidant activity of ethyl-acetate fraction had the greatest antioxidant activity with the IC50 value 54.89 μg /mL while the highest antimicrobial efficacy of the ethyl acetate extract (3.125, 6.25, 12.5, 25, 50 and 100 µg/ml) was demonstrated by the inhibitory zones (12-19 mm for Staphylococcus- aureus,16-20 mm for E. coli,16-21 mm for candida albicans) compared with positive control Augmentin (19 mm) and ketoconazole (16mm) respectively

Conclusion: The first identification of antimicrobial and antioxidant activity of E. japonicus in vitro ,show this plant have highest activity in compared to standard, this activity related to the polyphenolic compound .

Received Oct. 2022

Accepted May 2023

Published July,2023

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References

1. ames T. Japanese spindle tree Euonymus japonicus. [cited 2022 May 13]; Available from: https://www.chewvalleytrees.co.uk/products/detail/euonymus-japonica

Ruiz-Riaguas A, Fernández-de Córdova ML, Llorent-Martínez EJ. Phenolic profile and antioxidant activity of Euonymus japonicus Thunb. Nat Prod Res. 2022;36(13):3445-9.

Bensid A, El Abed N, Houicher A, Regenstein JM, Özogul F. Antioxidant and antimicrobial preservatives: Properties, mechanism of action and applications in food-a review. Crit Rev Food Sci Nutr. 2022;62(11):2985-3001.

https://doi.org/10.1080/10408398.2020.1862046

Tian J, Walayat N, Ding Y, Liu J. The role of trifunctional cryoprotectants in the frozen storage of aquatic foods: Recent developments and future recommendations. Compr Rev Food Sci Food Saf. 2022;21(1):321-39.

https://doi.org/10.1111/1541-4337.12865

Crepaldi AL, da Rocha Bispo AS, Cruz DCB, Tavechio WLG, Araújo FM, Rocha TVS, et al. Phytochemical screening, toxicity and antimicrobial activity of different Mimosa tenuiflora extracts on Aeromonas strains. Semin Ciências Agrárias. 2022;43(2):641-56.

https://doi.org/10.5433/1679-0359.2022v43n2p641

Lama-Muñoz A, del Mar Contreras M, Espínola F, Moya M, Romero I, Castro E. Content of phenolic compounds and mannitol in olive leaves extracts from six Spanish cultivars: Extraction with the Soxhlet method and pressurized liquids. Food Chem. 2020;320:126626.

https://doi.org/10.1016/j.foodchem.2020.126626

Kim S-H, Yoon KD. HPLC Method Validation for Quantitative Analysis of Scopoletin from Hot-Water Extract Powder of Artemisia annua Linné. Korean J Pharmacogn. 2020;51(1):78-85.

Nandhini S, Ilango K. Simultaneous Quantification of Lupeol, Stigmasterol and β-Sitosterol in Extracts of Adhatoda vasica Nees Leaves and its Marketed Formulations by a Validated RP-HPLC Method. Pharmacogn J. 2020;12(4).

https://doi.org/10.5530/pj.2020.12.122

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181(4617):1199-200.

https://doi.org/10.1038/1811199a0

Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem. 2005;53(10):4290-302. https://doi.org/10.1021/jf0502698

Hazrati S, Nicola S, Khurizadeh S, Alirezalu A, Mohammadi H. Physico-chemical properties and fatty acid composition of Chrozophora tinctoria seeds as a new oil source. Grasas y Aceites. 2019;70(4):e328-e328. https://doi.org/10.3989/gya.0939182

Oke F, Aslim B, Ozturk S, Altundag S. Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten. Food Chem. 2009;112(4):874-9. https://doi.org/10.1016/j.foodchem.2008.06.061

Ojha D, Maity C, Mohapatra P Das, Samanta A. In vitro antimicrobial potentialities of different solvent extracts of ethnomedicinal plants against clinically isolated... J Phytol. 2010;2(4):57-64.

Muttalib LY, Naqishbandi AM. Antibacterial and phytochemical study of Iraqi Salvia officinalis leave extracts. Iraqi J Pharm Sci (P-ISSN 1683-3597, E-ISSN 2521-3512). 2012;21(1):93-7. https://doi.org/10.31351/vol21iss1pp93-97

Huber U, Majors RE. Principles in preparative HPLC. Agil Technol Inc, Ger. 2007;2:60-71.

Gong Z, Chen S, Gao J, Li M, Wang X, Lin J, et al. Isolation and purification of seven catechin compounds from fresh tea leaves by semi-preparative liquid chromatography. Se pu= Chinese J Chromatogr. 2017;35(11):1192-7.

https://doi.org/10.3724/SP.J.1123.2017.08002

Suh H-J, Kim S-R, Lee K-S, Park S, Kang SC. Antioxidant activity of various solvent extracts from Allomyrina dichotoma (Arthropoda: Insecta) larvae. J Photochem Photobiol B Biol. 2010;99(2):67-73.

https://doi.org/10.1016/j.jphotobiol.2010.02.005

Syaputri I, Girsang E, Chiuman L. Test Of Antioxidant And Antibacterial Activity Of Ethanol Extract Of Andaliman Fruit (Zanthoxylum Acanthopodium Dc.) With Dpph (1.1-Diphenyl-2-Picrylhydrazil) Trapping Method And Minimum Inhibitory Concentration. Int J Heal Pharm. 2022;2(2):215-24. https://doi.org/10.51601/ijhp.v2i2.36

Emran T Bin, Rahman MA, Uddin MMN, Dash R, Hossen MF, Mohiuddin M, et al. Molecular docking and inhibition studies on the interactions of Bacopa monnieri's potent phytochemicals against pathogenic Staphylococcus aureus. DARU J Pharm Sci. 2015;23(1):1-8.

https://doi.org/10.1186/s40199-015-0106-9

Hultmark, Dan. "Quantification of antimicrobial activity, using the inhibition-zone assay." (1998): 103-107.‏

Hussein AA, Saour KY. Extraction, Identification and Isolation of B-sitosterol from Iraqi Wild Awsaj plant (Lycium barbarum) Using UAE (Probe and Bath) and two isolation technique (HPTLC and PHPLC): B-sitosterol in Iraqi Awsaj plant. Iraqi J Pharm Sci (P-ISSN 1683-3597, E-ISSN 2521-3512). 2017;75-84. https://doi.org/10.31351/vol26iss2pp75-84

Babaei F, Moafizad A, Darvishvand Z, Mirzababaei M, Hosseinzadeh H, Nassiri‐Asl M. Review of the effects of vitexin in oxidative stress‐related diseases. Food Sci Nutr. 2020;8(6):2569-80. https://doi.org/10.1002/fsn3.1567