Antioxidant Effects of Selenium Nanoparticles Prepared from Eruca Sativa Extract on Ketoconazole –Induced Testicular Oxidative Damage in Male Rats
DOI:
https://doi.org/10.32007/jfacmedbagdad.6612174Keywords:
Glutathione peroxidase, Malondialdehyde, Nano Selenium, Oxidative stress, Superoxide dismutase, SeleniumAbstract
Background: Recent studies have shown that the anti-fertility effects of Ketoconazole can be minimized by taking an antioxidant nanoparticles based-plant extract in combination with the medication or by taking it after medication. One of the effective methods to improve its medicinal properties and decrease the toxicities of Selenium is by formulating it as nanoparticles. However, this is still a challenging strategy.
Objectives: The aim of this study was to analyze the impact of nano selenium, which was prepared from Eruca Sativa extract, on testicular oxidative stress parameters in rats that were treated with ketoconazole. The study will focus on the evaluation of glutathione peroxidase, superoxide dismutase, and malondialdehyde as indicators of oxidative stress.
Methods: In this study, a 1% w/v solution of plant extract was added to a solution of 10mM sodium selenite in different ratios, and placed on a magnetic stirrer in the dark for 12 hours at a temperature 50Cº and pH9. The solution was then left for 48 hours, and the optimal fabricated selenium nanoparticles were selected for further characterization. forty-eight rats were divided into six groups with eight animals in each. Group A was the negative control, while Group B was given oral Ketoconazole at a dose of 50mg/kg for fourteen days. Group BC, BD, BE1 and BE2 were given oral Ketoconazole at a dose of 50 mg/kg for fourteen days, followed by 200mg/kg Eruca Sativa, 0.5mg/kg oral sodium selenite, 0.25mg/kg oral nano selenium and 0.5mg/kg oral nano selenium for 28 days, respectively. Finally, the animals were euthanized and their testicle anti-oxidant parameters were evaluated.
Results: Significant increases in glutathione peroxidase and superoxide dismutase ( p value < 0.001), and a decrease in malondialdehyde levels ( p value < 0.001) were observed in groups treated with nanoparticles compared to control group.
Conclusion: Nano forms of prepared by Eruca sativa extract exhibit significant antioxidant effects on testicular tissues, while being available for several metabolic, biological, and physiological functions.
Received: Jun,, 2023
Accepted: Nov 2023
Published: Aprl.2024
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References
Alsalman ARS, Almashhedy LA, Hadwan MH. Effect of Oral Zinc Supplementation on the Thiol Oxido-Reductive Index and Thiol-Related Enzymes in Seminal Plasma and Spermatozoa of Iraqi Asthenospermic Patients. Biol Trace Elem Res. 2018; 184(2):340-9. https://doi.org/10.1007/s12011-017-1215-8
Landis-Piwowar KR, Iyer NR. Cancer chemoprevention: current state of the art. Cancer Growth Metastasis. 2014; 7:19-25. https://doi.org/10.4137/CGM.S11288
Salman FS, Al-Qadhi HI, Al Kareem BA. N-acetyl cysteine's effect on semen parameters in a sample of Iraqi men with oligoasthenoteratozoospermia. JFac Med Baghdad. 2022;64(3):170-4. https://doi.org/10.32007/jfacmedbagdad.6431938
ASSUMAIDAEE AAM, ALI NM, IBRAHEEM ZO, FADHIL AA, AL-HELLI KAA. Anti-Oxidoreductive stress Effects of Iraqi Olea Europaea L. Leaves Extract against Low Double Doses of Alloxan Induced Diabetes Mellitus in Rats. SRP. (2020),11(1): 292-302. https://doi.org/10.5530/srp.2020.1.38
Habbal H, Karabet F. Chitosan-(Prunus avium) Gum Nanocapsules Loaded with Orange Peel Extract: Nanocapsules Loaded with Orange Peel Extract. Iraqi Journal of Pharmaceutical Sciences. 2023; 32(1):194-201.
https://doi.org/10.31351/vol32iss1pp194-201
Patra JK, Das G, Fraceto LF, Campos EVR, Rodriguez-Torres MDP, Acosta-Torres LS, et al. Nano based drug delivery systems: recent developments and future prospects. J Nanobiotechnology. 2018;16(1):71.
https://doi.org/10.1186/s12951-018-0392-8
Khamees EJ. Nanotechnology and Its Applications in Disease Diagnosis, Drug Delivery, and Medical Imaging . JUBPAS [Internet]. 2021 Dec. 1 [cited 2024 Mar. 29];29(3):196-20. https://journalofbabylon.com/index.php/JUBPAS/article/view/3902
Hamza AA, Gamel M, Abdalla A, Abdalla Y, Amin A. Gentiana lutea attenuates hepatotoxicity induced by ketoconazole in rats by fortifying the cellular antioxidant defense system. The Journal of Basic and Applied Zoology. 2023;84(1):1-12. https://doi.org/10.1186/s41936-022-00321-7
Semet M, Paci M, Saïas-Magnan J, Metzler-Guillemain C, Boissier R, Lejeune H, et al. The impact of drugs on male fertility: a review. Andrology. 2017; 5(4):640-63. https://doi.org/10.1111/andr.12366
Al-Okaily BN. Role of alcoholic extract of Roket (Eruca sativa) leaves on male reproduction of experimentally induced-oxidative stressed rats: Baraa Najim AL-Okaily and Ahmed Jasim Nowfel. The Iraqi Journal of Veterinary Medicine. 2015;39(2):47-54. https://doi.org/10.30539/iraqijvm.v39i2.177
Ibraheem ZO, Satar M, Abdullah NA, Rathore H, Tan YC, Uldin F, et al. Antioxidant and cardio protective effect of palm oil leaves extract (standardized ethanolic fraction) in rats' model of saturated fats induced metabolic disorders. Pak J Pharm Sci. 2014;27(1):1-9. https://pubmed.ncbi.nlm.nih.gov/24374430/
Ibraheem ZO, Sattar MA, Abdullah NA, Hassan R, Johns EJ. Toxicity, phytochemical content and antioxidant activity assessment studies for standardized ethanolic fraction of palm oil leaf extract. Pharmacogn Commun. 2012;2:21-30. https://doi.org/10.5530/pc.2012.1.5
Nhon Hoang TN, Phan TT, Lien Phan TK, Van Nguyen NH, Dao Dong TA, Anh Le TH. Phytochemical Screening, Extraction, and Determination of the Bioactivities of the Extract-Enriched Polyphenols and Saponins from Musa balbisiana Fruit. Journal of Food Processing and Preservation. 2023;2023. https://doi.org/10.1155/2023/2581641
Pyrzynska K, Sentkowska A. Biosynthesis of selenium nanoparticles using plant extracts. Journal of Nanostructure in Chemistry. 2021:1-14. https://doi.org/10.1007/s40097-021-00435-4
Naidu ECS, Olojede SO, Lawal SK, Rennie CO, Azu OO. Nanoparticle delivery system, highly active antiretroviral therapy, and testicular morphology: The role of stereology. Pharmacol Res Perspect. 2021;9(3):e00776. https://doi.org/10.1002/prp2.776
Anbu J, Mahendra SA, Sundar LM, Reddy RP, Nayak D. Evaluation of antifertility potential of Ketoconazole and assessment of its reversible effect on concomitant treatment with Metformin and Hydroxytyrosol in rats. Asian Journal of Pharmacy and Pharmacology. 2019;5(1):1-15. https://doi.org/10.31024/ajpp.2019.5.1.1
Abd-Elsalam RM, El Badawy SA, Ogaly HA, Ibrahim FM, Farag OM, Ahmed KA. Eruca sativa seed extract modulates oxidative stress and apoptosis and up-regulates the expression of Bcl-2 and Bax genes in acrylamide-induced testicular dysfunction in rats. Environ Sci Pollut Res Int. 2021;28(38):53249-66. https://doi.org/10.1007/s11356-021-14532-y
Khalaf AA, Ahmed W, Moselhy WA, Abdel-Halim BR, Ibrahim MA. Protective effects of selenium and nano-selenium on bisphenol-induced reproductive toxicity in male rats. Hum Exp Toxicol. 2019;38(4):398-408. https://doi.org/10.1177/0960327118816134
Vyas R, Kesari KK, Slama P, Roychoudhury S, Sisodia R. Differential Activity of Antioxidants in Testicular Tissues Following Administration of Chlorophytum borivilianum in Gamma-Irradiated Swiss Albino Mice. Front Pharmacol. 2021;12:774444. https://doi.org/10.3389/fphar.2021.774444
Dmour I. Physicochemical Characterization of Nanobiocomposites. InBiocomposites-Recent Advances 2022 Dec 23. IntechOpen. https://doi.org/10.5772/intechopen.108818
Jay V, Shafkat R. Synthesis of selenium nanoparticles using Allium sativum extract and analysis of their antimicrobial property against gram positive bacteria. The Pharma Innovation. 2018;7(9):262-6. https://www.thepharmajournal.com/archives/2018/vol7issue9/PartE/7-9-34-545.pdf
Titus D, James Jebaseelan Samuel E, Roopan SM. Chapter 12 - Nanoparticle characterization techniques. In: Shukla AK, Iravani S, editors. Green Synthesis, Characterization and Applications of Nanoparticles: Elsevier; 2019. p. 303-19. https://doi.org/10.1016/B978-0-08-102579-6.00012-5
Mohammed RA, Saleh GM, Mutlak FA. Investigate the Structural Properties of Silver Nanoparticles Produced by Bio Production of Green Spinicia Oleracea Leaf Extract and their Influence on Antibacterial Activity. Iraqi Journal of Nanotechnology. https://www.iasj.net/iasj/download/0ee156c72c87b303
Cittrarasu V, Kaliannan D, Dharman K, Maluventhen V, Easwaran M, Liu WC, et al. Green synthesis of selenium nanoparticles mediated from Ceropegia bulbosa Roxb extract and its cytotoxicity, antimicrobial, mosquitocidal and photocatalytic activities. Sci Rep. 2021;11(1):1032. https://doi.org/10.1038/s41598-020-80327-9
Ramamurthy C, Sampath KS, Arunkumar P, Kumar MS, Sujatha V, Premkumar K, et al. Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioprocess Biosyst Eng. 2013;36(8):1131-9. https://doi.org/10.1007/s00449-012-0867-1
Zambonino MC, Quizhpe EM, Jaramillo FE, Rahman A, Santiago Vispo N, Jeffryes C, et al. Green Synthesis of Selenium and Tellurium Nanoparticles: Current Trends, Biological Properties and Biomedical Applications. Int J Mol Sci. 2021;22(3). https://doi.org/10.3390/ijms22030989
Bibi I, Nazar N, Ata S, Sultan M, Ali A, Abbas A, et al. Green synthesis of iron oxide nanoparticles using pomegranate seeds extract and photocatalytic activity evaluation for the degradation of textile dye. Journal of materials research and technology. 2019;8(6):6115-24. https://doi.org/10.1016/j.jmrt.2019.10.006
Jasim AS, Salman ON. The Effect of Solvent Variation on Structural, Optical, and Electrical Properties of TiO2 Films Prepared by Hydrothermal Method. Journal of Applied Sciences and Nanotechnology. 2023;3(2):59-69. https://doi.org/10.53293/jasn.2023.5562.1191
Skandalis N, Dimopoulou A, Georgopoulou A, Gallios N, Papadopoulos D, Tsipas D, et al. The Effect of Silver Nanoparticles Size, Produced Using Plant Extract from Arbutus unedo, on Their Antibacterial Efficacy. Nanomaterials (Basel). 2017;7(7). https://doi.org/10.3390/nano7070178
Danaei M, Dehghankhold M, Ataei S, Hasanzadeh Davarani F, Javanmard R, Dokhani A, et al. Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems. Pharmaceutics. 2018;10(2). https://doi.org/10.3390/pharmaceutics10020057
Kmkm AM, Ghareeb MM. Natural Oil Nanoemulsion-Based Gel Vehicle for Enhancing Antifungal Effect of Topical Luliconazole. JFac MedBaghdad. 2023; 65(1):65-73. https://doi.org/10.32007/jfacmedbagdad.6512058
Shim G, Jeong S, Oh JL, Kang Y. Lipid-based nanoparticles for photosensitive drug delivery systems. J Pharm Investig. 2022;52(2):151-60. https://doi.org/10.1007/s40005-021-00553-9
Sadeghi B, Gholamhoseinpoor F. A study on the stability and green synthesis of silver nanoparticles using Ziziphora tenuior (Zt) extract at room temperature. Spectrochim Acta A Mol Biomol Spectrosc. 2015;134: 310-5. https://doi.org/10.1016/j.saa.2014.06.046
Kadhim WK, Alshmmari MK, Al-Ethary MA. Synthesis and Characteristics of (PAV-PAVC-Ti) Nanocomposites. Iraqi Journal of Nanotechnology, synthesis and application. 2020; 1: 21-6. https://doi.org/10.47758/ijn.vi1.25
Nandiyanto ABD, Oktiani R, Ragadhita R. How to read and interpret FTIR spectroscope of organic material. Indonesian Journal of Science and Technology. 2019; 4(1):97-118. https://doi.org/10.17509/ijost.v4i1.15806
Petricca S, Carnicelli V, Luzi C, Cinque B, Celenza G, Iorio R. Oxidative Stress, Cytotoxic and Inflammatory Effects of Azoles Combinatorial Mixtures in Sertoli TM4 Cells. Antioxidants. 2023; 12(6):1142.
https://doi.org/10.3390/antiox12061142
El-Sharawy ME, Hamouda M, Soliman AA, Amer AA, El-Zayat AM, Sewilam H, et al. Selenium nanoparticles are required for the optimum growth behavior, antioxidative capacity, and liver wellbeing of Striped catfish (Pangasianodon hypophthalmus). Saudi J Biol Sci. 2021; 28(12):7241-7.
https://doi.org/10.1016/j.sjbs.2021.08.023
Nateq S, Moghaddam G, Alijani S, Behnam M. The effects of different levels of Nano selenium on the quality of frozen-thawed sperm in ram. Journal of Applied Animal Research. 2020;48(1):434-9.
https://doi.org/10.1080/09712119.2020.1816549
Rajput VD, Harish, Singh RK, Verma KK, Sharma L, Quiroz-Figueroa FR, et al. Recent Developments in Enzymatic Antioxidant Defence Mechanism in Plants with Special Reference to Abiotic Stress. Biology (Basel). 2021;10(4). https://doi.org/10.3390/biology10040267
Adhikari A, Mondal S, Das M, Ghosh R, Biswas P, Darbar S, et al. Redox Buffering Capacity of Nanomaterials as an Index of ROS-based Therapeutics and Toxicity: A Preclinical Animal Study. bioRxiv. 2021:2021.03.14.435286. https://doi.org/10.1101/2021.03.14.435286
Asri-Rezaei S, Nourian A, Shalizar-Jalali A, Najafi G, Nazarizadeh A, Koohestani M, et al. Selenium supplementation in the form of selenium nanoparticles and selenite sodium improves mature male mice reproductive performances. Iran J Basic Med Sci. 2018; 21(6):577-85. https://doi.org/10.22038/IJBMS.2018.26023.6397.
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