Molecular Detection and Genotyping of Human Papilloma Virus Infections in Iraqi Patients with Esophageal Carcinoma.

Authors

  • Intisar M. Jumaa College of Medical and Health Technologies
  • Saad H. Mohammed Ali College of Medicine / University of Baghdad
  • Tural Y. Bakir College of Medical and Health Technologies
  • Delaram M. M. Ardalan Teaching laboratories / Medical City

DOI:

https://doi.org/10.32007/jfacmedbagdad.5121214

Keywords:

Esophageal Carcinoma; Human Papilloma Virus; In Situ Hybridization.

Abstract

Background: Molecular DNA hybridization has confirmed more than 120 different human papilloma virus (HPV) genotypes. A small group of them have high- risk oncogenic potential. Many studies have described an association of such high risk-HPV genotypes with a variety of esophageal benign tumors as well as malignant squamous cell carcinomas.
Patients and Methods: A total number of 90 tissue specimens were collected from 50 patients with esophageal squamous cell (SCC), adenocarcinoma (AC) and carcinoma in situ (CIS); 20 patients with squamous acanthosis (SA); and 20 individuals with apparently-healthy esophageal tissues (AHET). The molecular detection methods for HPV detection and genotyping were performed by in situ hybridization using cocktailed- and specific high- risk HPV DNA probes, respectively.
Results: The overall percentage of HPV in the total group of esophageal carcinoma was 20%.The percentage of HPV DNA in the subgroup of SCC and AC was 26.7% and 13.3%, respectively,. However, neither HPV DNA was detected in CIS subgroup nor in both control groups (SA and AHET).The overall genotyping results showed that HPV 18 constituted the majority of the detected high-risk oncogenic HPV genotypes, followed by HPV 16 then HPV 31/33.
Conclusions: Despite the low prevalence of HPV infection and rarity of invasive esophageal carcinoma in the general Iraqi population, the detection of high percentage of such high oncogenic risk- HPV genotypes in these carcinomas indicating for a relevant importance in esophageal carcinogenesis.

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Published

01.07.2009

How to Cite

1.
Jumaa IM, Mohammed Ali SH, Bakir TY, Ardalan DMM. Molecular Detection and Genotyping of Human Papilloma Virus Infections in Iraqi Patients with Esophageal Carcinoma. J Fac Med Baghdad [Internet]. 2009 Jul. 1 [cited 2024 Nov. 14];51(2):204-10. Available from: https://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1214

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