استخراج وتحديد المكونات الرئيسية لمستخلص زيت القرنفل (Syzygium Aromaticum L.) ونشاطه المضاد للميكروبات ضد جرثومة المكورات العنقودية الذهبية المقاومة للميثيسيلنMRSA.
DOI:
https://doi.org/10.32007/jfacmedbagdad.6622187الكلمات المفتاحية:
Antimicrobial، Caryophyllen، Clove extract، Staphylococcus aureus (MRSA)، Time killing curve.الملخص
خلفية: تزايد استخدام الأدوية العشبية بدلاً من الأدوية التقليدية نتيجة مقاومة المضادات الحيوية ، مما يشكل خطراً على الصحة العامة.
الأهداف: دراسة التركيب الكيميائي واخنبارالنشاط المضاد للبكتيريا في المختبر لمستخلص زيت القرنفل ضد جرثومة المكورات العنقودية الذهبية المقاومة للميثيسيلن MRSA)) .
الطريقة: تم الحصول على مستخلص زيت القرنفل باستخدام التقطير المائي بواسطة جهاز Clevenger. بعد ذلك ، تم إجراء التحليل الكيميائي النباتي عن طريق التحليل الكروماتوجرافي-مطياف الكتلة. تم إجراء فحص في المختبر لمضادات الميكروبات لزيت القرنفل باستخدام طريقة انتشار القرص المزدوج ، وطريقة التخفيف الدقيق ، وحركة منحنى وقت القتل في المختبر.
النتائج: بلغت نسبة استخلاص مستخلص القرنفل 50٪. وقد أظهر التحليل الكيميائي النباتي وجود مكونات مختلفة وبشكل رئيسي هي Caryophyllen و Humulene و Eugenol. حيث تبين ان المكورات العنقودية الذهبية كانت حساسة لمستخلص القرنفل بتركيزات مختلفة علاوة على ذلك ، وجد أن أقل تركيز مثبط (MIC) وأقل تركيز مبيد للجراثيم (MBC) كان 2.5 ميكروغرام / مل ، 5.0 ميكروغرام / مل على التوالي.
الاستنتاجات: أظهرت الدراسة الحالية أن مستخلص القرنفل أظهر نشاطًا جيدًا مضادًا للبكتيريا في المختبر
التنزيلات
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