التأثير السام للخلايا الغليوتوكسن من صنف المبيِّضات المعزولة من مصادر سريرية ضد السرطان وخطوط الخلايا الطبيعية

المؤلفون

DOI:

https://doi.org/10.32007/jfacmedbagdad.2078

الكلمات المفتاحية:

Candida albicans، gliotoxin، HPLC، cytotoxicity

الملخص

خلفية البحث: أصبحت العدوى الفطرية الغازية أكثر شيوعًا خلال العقدين الماضيين. أنواع المبيضات، هي أكثر أنواع العدوى الفطرية البشرية شيوعًا. يتميز بإصابات داخلية بسبب عوامل الضراوة، مثل سم الجليوتوكسين وهو نوع من السموم الفطرية التي يعتقد أنها مضادة للبكتيريا والفطريات والفيروسات.

هدف الدراسة: كان الهدف من الدراسة الحالية هو اختبار قدرة أنواع المبيضات المعزولة من المصادر السريرية على إنتاج سم الجليوتوكسين كعامل ضراوة والتحقيق في تأثيرات السمية الخلوية ضد بعض سلالات الخلايا المختارة.

المواد والطرق: تم جمع 110 عزلة سريرية من أنواع المبيضات، وتحديدها وتشخيصها من خلال طرق المختبر الروتينية والاوساط الزرعية. تم اختبار وقياس قدرة عزلات المبيضات على إنتاج سم الجليوتوكسين بالطرق التحليلية. تم تطبيق السمية الخلوية للسم الجليوتوكسين الناتج ضد سلالات الخلايا الطبيعية والسرطانية.

النتائج: تم تحديد 110 عزلة على النحو التالي، المبيضات البيضاء، المبيضات الاستوائية، داء المبيضات parapsilosis  Candida glabrata, Candida Krusei, Candida kefyr, Candida, lusitaniae,  Candida rugosa, . 28 عزلة من المبيضات أظهرت إنتاج السموم الدبقية، وتم الإبلاغ عن تأثيرات السمية الخلوية للسم الجليوتوكسين الخلايا الليمفاوية وخطوط الخلايا AMGM و AMJ13 بتركيزات مختلفة. لوحظ أعلى تأثير سام للخلايا بتركيز 400 ميكروغرام / مل من السم الجليوتوكسين.

الاستنتاجات: أشارت النتائج إلى أن إمراضية الكانديدا كانت موزعة على جميع الأعمار من الجنسين وأنواع عديدة من العزلات الإكلينيكية. بعض العزلات كانت لديها القدرة على إنتاج سم الجليوتوكسين، كما أظهرت النتائج تأثير السم على الخلايا الطبيعية والسرطانية

التنزيلات

تنزيل البيانات ليس متاحًا بعد.

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التنزيلات

منشور

2023-10-01

كيفية الاقتباس

1.
مهدي ناع, Shanter Al-Qaysi SA-DA. التأثير السام للخلايا الغليوتوكسن من صنف المبيِّضات المعزولة من مصادر سريرية ضد السرطان وخطوط الخلايا الطبيعية. J Fac Med Baghdad [انترنت]. 1 أكتوبر، 2023 [وثق 19 يناير، 2025];65(3):212-9. موجود في: https://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/2078

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