Comparison of Pharmacokinetic Characteristics of Bilosomal Dispersion Versus Pure Solution of Oral Ropinirole Hydrochloride in Rats




Bilosomes; , Bioavailability;, nanovesicular carriers; , Pharmacokinetics;, Ropinirole hydrochloride.


Background: Ropinirole hydrochloride is a non-ergoline antiparkinson drug. It is a highly hydrophilic drug and classified as class III according to Biopharmaceutical Classification System with low absolute oral bioavailability of approximately 50% upon oral administration due to significant hepatic first-pass metabolism.

Objective: to compare the pharmacokinetic parameters of Ropinirole,  when administered orally in the form of a Ropinirole bilosomal dispersion in contrast to an oral Ropinirole solution.

Methods: This study involved the use of twelve male Wistar rats, with an average weight of 220±11 g, and these rats were divided into two groups, comprising six rats each. A 1.1mg/kg doses of pure Ropinirole and Ropinirole bilosomes were administered orally through gavage after reconstituting in distilled water. Ropinirole was quantified in the rat's plasma using HPLC, subsequently establishing a spiked calibration curve with plasma samples and utilizing paracetamol as an internal standard. The statistics included mean values (± SD; n = 6) for pharmacokinetic parameters, with statistical significance assessed using a Student's t-test.

Results: For the oral bilosomes, the values were 9.4±0.11 μg /ml for Cmax, 3±0.00 h for Tmax, and 55.56±2.12 μg h/ml for AUC0-24. In contrast, for the oral solution, the corresponding values were 7.2±0.14 μg/ml for Cmax, 1.5±0.00 h for Tmax, and  23.70±2.23 μg h/ml for AUC0-24. These parameters were significantly higher (P<0.05) as compared with a pure drug solution. The comparative bioavailability of Ropinirole (AUC0-24 oral solution / AUC0-24 oral bilosomes ) is equal to 42.66%, which indicates the bioavailability of the oral RH solution was less than that of RH bilosomal dispersion.

Conclusions: The use of nano vesicular carriers (bilosomes) shows significant potential as an effective delivery system for improving the oral bioavailability of ropinirole hydrochloride


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Author Biographies

  • Samer K. Ali, Department of Pharmaceutics College of Pharmacy, University of Baghdad, Baghdad, Iraq.

    MSc Student 

  • Entidhar J. Al-Akkam, Department of Pharmaceutics College of Pharmacy, University of Baghdad, Baghdad, Iraq.



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Bilosomes, Bioavailability, nanovesicular carriers, Pharmacokinetics,  Ropinirole hydrochloride.




How to Cite

Ali SK, J. Al-Akkam E. Comparison of Pharmacokinetic Characteristics of Bilosomal Dispersion Versus Pure Solution of Oral Ropinirole Hydrochloride in Rats. J Fac Med Baghdad [Internet]. 2024 Jul. 1 [cited 2024 Jul. 13];66(2):201-8. Available from:

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