Study of ATPase and GTPase levels in Fibrotic Lung Disease with and without COVID-19 Vaccination

Raed M.   Al-Azawee; Zeinab M.  Al-Rubaei

doi:10.32007/jfacmedbagdad.6622272

Authors

Raed M. Al-Azawee Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, Baghdad, Iraq. https://orcid.org/0009-0006-3778-0970
Zeinab M. Al-Rubaei Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad , Baghdad, Iraq. https://orcid.org/0000-0002-2742-3068

DOI:

https://doi.org/10.32007/jfacmedbagdad.6622272

Keywords:

ATPase;, GTPase;, , COVID-19 Vaccination; , Fibrotic Lung Disease.

Abstract

Background: In eukaryotic cells, the acidification of intracellular compartments is the responsibility of vacuolar H+ ATPase, a family of proton pumps, sometimes known as V-ATPases. Small GTPases are signaling molecules that regulate important cellular processes as well as subcellular activities making them essential players, particularly in a wide variety of coronavirus infection processes.

Objectives: The purpose of this research was to assess the levels of ATPase and GTPase in fibrotic lung disease patients who had received or had not received the COVID-19 vaccination, and then to compare these levels with those of the control group.

Methods: A total of 150 individuals participated in this study, divided into three groups. The first group was the control group (N=50). In the second group (N=50) patients with fibrotic lung disease () who did not get the COVID-19 vaccination. Fifty patients who had received the COVID-19 vaccination made up the third group ()(N=50). Enzyme-linked immunosorbent assay was the method that was used to determine the amounts of ATPase and GTPase. The P- P-value of 0.05 or less is considered statistically significant. ROC tests were examined for ATPase and GTPase.

Results: The data analysis reported a significant rise in alkaline phosphatase, Alanine aminotransferase, and Aspartate-aminotransferase () among the three groups. Both ATPase and GTPase levels were shown to have significantly increased in Groups 3 and 2 as compared to Group 1 levels. Moreover, a substantial rise was discovered in Group 3 in comparison to Group 2 which was detected.

Conclusion: ATPase and GTPase levels are increased in patients with fibrotic lung disease regardless of the COVID-19 vaccination state.

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Author Biographies

Raed M. Al-Azawee, Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, Baghdad, Iraq.

University of Baghdad
Zeinab M. Al-Rubaei , Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad , Baghdad, Iraq.

Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad , Baghdad, Iraq.

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