Optimum Conditions Of 5-Nucleotidase Activity In Sera Of KalaAzaric Patients.
DOI:
https://doi.org/10.32007/jfacmedbagdad.4831513Keywords:
5 -Nuceotidase, serum and kala-azar.Abstract
Background: This research focuses on the characterization studies of 5v-Nucleotidase (5'-NT) of kalaazar patients' sera. 5V NT is one of the enzymes used as indicator of liver function test . In this study the optimum conditions for the activity of 5s - NT enzyme has been demonstrated for kalaazaric patients.
Materials and methods: Thirty five blood samples were obtained from Baghdad Hospitals from untreated infants and children with kala-azar. 5'-NT activity was measured by following the
method of Wood and Williams.
Results: Optimum conditions of serum 5' - NT activity of kala- azaric patients obtained were 50 u L , 30 minutes ,0.8 mM,7.7 and 50'C) as (Volume of serum , incubation time , substrate
concentration, pH and temperature) respectively
Downloads
Downloads
Published
Issue
Section
License
For all articles published in Journal of the Faculty of Medicine Baghdad, copyright is retained by the authors. Articles are licensed under an open access Creative Commons CC BY NC 4.0 license, meaning that anyone may download and read the paper for free. In addition, the article may be reused and quoted provided that the original published version is cited. These conditions allow for maximum use and exposure of the work, while ensuring that the authors receive proper rights.