The expression of Matrix metalloproteinase-2 and cyclooxygenase-2 by immunohistochemistry in patients with colorectal carcinoma

Areej A. Hussein; Basim M. Ibrahim; Basim M. Kashman

doi:10.32007/jfacmedbagdad.533834

Authors

Areej A. Hussein Dept. of Microbiology, College of Medicine, Diyala Unversity.
Basim M. Ibrahim Dept. of Oral Pathology, College of Dentist, University of Baghdad.
Basim M. Kashman Dept. of Microbiology, College of Medicine, University of Baghdad.

DOI:

https://doi.org/10.32007/jfacmedbagdad.533834

Keywords:

Colorectal neoplasm, Matrix metalloproteinase-2, Cyclooxygenase-2, Metastasis

Abstract

Background: Human colorectal carcinogenesis is a complex, multistep and multigenetic process. Matrix metalloproteinase-2 and cyclooxygenase-2 are key enzyme in degradation of extracellular material, are over expressed in several epithelia like colorectal adenocarcinoma.
Objectives: This study was designed to detect the expression of matrix metalloproteinase-2 and cyclooxygenase-2 in patients with colorectal carcinoma and their correlation with age, gender, tumor grade and presence or absence of muscle invasion.
Materials and methods: Immunohistochemical staining of MMP-2 and COX-2 was determined in 40 tissue samples from colorectal patients, from teaching laboratories in Baghdad medical city. In addition twelve apparently normal colorectal autopsies were used as a control group.
Results: The expression of matrix metalloproteinase-2 was detected in 30 of 40 colorectal cancer cases (75%) while cyclooxygenase-2 was detected in 31 0f 40 colorectal cancer cases (77.5%). All normal colorectal specimens showed negative results. The incidence of both of them was higher for grade II than those in earlier grade.
Conclusion: Cancer cells to have a more aggressive behavior may show an up regulation of tumor cell derived matrix metalloproteinase-2 and cyclooxygenase-2.