Novel methods to design wild bacteriophages into highly lytic and therapeutic bacteriophages to extensively drug-resistant Mycobacterium tuberculosis

Authors

  • Ahmed S. Abdulamir Dept. of Microbiology, College of Medicine, Alnahrain University.

DOI:

https://doi.org/10.32007/jfacmedbagdad.583264

Keywords:

bacteriophage, Mycobacterium tuberculosis, phage therapy, phage cocktail, phage designing, MDR bacteria.

Abstract

Background: The emergence of multiple drug resistant (MDR) Mycobacterium tuberculosis (M.TB) and extensive drug resistant (XDR) M.TB lay huge burden on TB endemic countries such as Iraq.
Objectives: Bacteriophage (phage) therapy can be used as alternative approach to tackle this problem. Patients and methods: Forty isolates of M.TB were cultured from TB-positive sputum specimens with three ATCC strains. Phage passaging and biokinetic based techniques were used to optimize wild anti-M.TB phages. Three chemical, non genetic- designing techniques, tween-80, mycobacterial lysis buffer, and xyelen, were used to change the specificity of wild anti-TB phages towards phage-resistant target M.TB.
Results: Five wild anti-M.TB phages were isolated and optimized. The optimization techniques were successful in enhancing plaques size, clarity, burst size, and infective ratio. Chemical designing techniques succeeded to reorient specificity of 6 phages to new host bacteria.
Conclusions: phage designing opens door wide for endless future applications of phage-based therapy, biocontrol and diagnosis.

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Published

2016-10-02

How to Cite

1.
Abdulamir AS. Novel methods to design wild bacteriophages into highly lytic and therapeutic bacteriophages to extensively drug-resistant Mycobacterium tuberculosis. JFacMedBagdad [Internet]. 2016 Oct. 2 [cited 2022 May 16];58(3):276-82. Available from: https://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/264