The Association between Leptin and Asprosin Levels in Female Patients with Type II Diabetes Mellitus

Duha Q. Bakr; Ahmed Y.  Abed

doi:10.32007/jfacmedbaghdad.6632346

Authors

Duha Q. Bakr Department of Chemistry, College of Sciences for Women, University of Baghdad, ‎Baghdad, Iraq.‎ https://orcid.org/0009-0009-4273-6685
Ahmed Y. Abed Department of Chemistry, College of Sciences for Women, University of Baghdad, ‎Baghdad, Iraq https://orcid.org/0000-0001-6678-3448

DOI:

https://doi.org/10.32007/jfacmedbaghdad.6632346

Keywords:

Asprosin, Fasting Blood Glucose, Glycated ‎hemoglobin, Leptin, Type II diabetes mellitus

Abstract

Background: Leptin and asprosin are adipokines secreted by white adipose tissue. The leptin and asprosin molecules have many functions in the central nervous system and other functions of the body: Appetite, glucose ‎metabolism, ‎insulin resistance, and cellular death.

Objectives: The study aims to determine the potential relationship between leptin and asprosin hormones in female patients with type II diabetes mellitus.

Methods: The present study was conducted in Al-Mahmodia Hospital / Baghdad and the laboratories of the College of Science for Girls / University of Baghdad / Iraq, for the period from 1/11/2023 to 1/2/ 2024. This study is a comparative analysis of several essential biomarkers ‎found in the sera of individuals with diabetes via estimating leptin, asprosin, fasting blood glucose, glycated ‎hemoglobin, body mass index, total cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase in females with type II diabetes mellitus. The study consisted of 60 participants grouped into: Group I (30 females ‎with diabetes), and group II (30 healthy females). The Biochemical ‎parameters of every participant were ascertained. The quantification of leptin and asprosin in the serum was conducted using the enzyme-linked immunosorbent assay (ELISA).

Results: The levels of leptin and asprosin were markedly high in the diabetic group [(‎5.1 ± 0.69‎‎), and (10.3 ± 1.07)] compared with the control group [(2.0 ± 0.48), and (1.6 ± 0.16)] respectively, with a significant difference.

Conclusion: Despite the high levels of leptin and asprosin in female patients with Type II diabetes mellitus, the relationship between leptin and asprosin was a weak negative one.

Received: April, 2024

Revised: June,2024

Accepted: July, 2024

Downloads

Download data is not yet available.

References

Lefta NA, Abed AY, Abed BA. Estimation of asprosin levels in female Iraqi patients with type 2 diabetes and hypothyroidism. J Med Chem Sci. 2023;6(2):433-9. https://doi.org/10.26655/JMCHEMSCI.2023.2.23

Alimoradzadeh R, Moosavi N, Karimkoshteh A, Sadeghi Z, Fard MM, Ismaili A. Investigation of the Chemistry of Metformin by Targeting the Nrf2 Signaling Pathway (A response Surface Methodology Approach). Chemical Methodologies. 2022;6(3):166-73. https://doi.org/10.22034/chemm.2022.315764.1395

Alwan LH, Khaleel FM, Hameed AS, Al-Ghani RH. Determination of Polymorphism of ‎Glutathione S Transferase (Gst) in the Iraqi (Diabetic and Non-Diabetic) ‎Acromegalic Patients. Biochemical & Cellular Archives. 2020 Oct 1;20(2). https://www.researchgate.net/profile/Luma

Al-Yassin HD. Correlation of Serum levels of Chromium, Copper, and Manganese with the Glucose levels in Type 2 Diabetes Mellitus in Iraq. Journal of the Faculty of Medicine Baghdad. 2023;65(4). https://doi.org/10.32007/jfacmedbagdad.2126.

Saeed WM, Binjawhar DN. Association of Serum Leptin and Adiponectin Concentrations with Type 2 Diabetes Biomarkers and Complications Among Saudi Women. Diabetes, Metab Syndr Obes. 2023;16:2129-2140. https://doi.org/10.2147/DMSO.S405476

Khaleel FM, Noor NO, Abed BA. Disturbance of Arginase Activity and Nitric Oxide Levels in Iraqi Type 2 Diabetes Mellitus. Baghdad Science Journal. 2018 Jun 4;15(2):0189-. https://doi.org/10.21123/bsj.2018.15.2.0189

Hekim MG, Kelestemur MM, Bulmus FG, Bilgin B, Bulut F, Gokdere E, et al. Asprosin, a novel glucogenic adipokine: a potential therapeutic implication in diabetes mellitus. Arch Physiol Biochem. 2023;129(5):1038-1044. https://doi.org/10.1080/13813455.2021.1894178

Ali SE, Khaleel FM, Ali FE. A study of apelin-36 and GST levels with their relationship to lipid and other biochemical parameters in the prediction of heart diseases in PCOS women patients. Baghdad Science Journal. 2020 Sep 8;17(3 (Suppl.)):0924-. https://doi.org/10.21123/bsj.2020.17.3(Suppl.).0924

Kumari R, Kumar S, Kant R. An update on metabolic syndrome: Metabolic risk markers and adipokines in the development of metabolic syndrome. Diabetes Metab Syndr Clin Res Rev. 2019;13(4):2409-2417.

https://doi.org/10.1016/j.dsx.2019.06.005

Farhan LO, Abed BA, Dawood A. Comparison study between adipsin levels in sera of Iraqi ‎patients with diabetes and neuropathy. Baghdad Science Journal. 2023 Jun 1;20(3):0726-. https://doi.org/10.21123/bsj.2022.7408

Ozcan S, Ulker N, Bulmus O, Yardimci A, Ozcan M, Canpolat S. The modulatory effects of irisin on asprosin, leptin, glucose levels, and lipid profile in healthy and obese male and female rats. Arch Physiol Biochem. 2022;128(3):724-731.

https://doi.org/10.1080/13813455.2020.1722706

Peng X, Huang J, Zou H, Peng B, Xia S, Dong K, et al. Roles of plasma leptin and resistin in novel subgroups of type 2 diabetes driven by cluster analysis. Lipids Health Dis. 2022;21(1):1-10. https://doi.org/10.1186/s12944-022-01623-z.

Katsiki N, Mikhailidis DP, Banach M. Leptin, cardiovascular diseases and type 2 diabetes mellitus review-article. Acta Pharmacol Sin. 2018;39(7):1176-1188. https://doi.org/10.1038/aps.2018.40

Onyemelukwe OU, Ogoina D, Onyemelukwe GC. Leptin concentrations in type 2 diabetes and non-diabetes Nigerian-Africans. Am J Cardiovasc Dis. 2020;10(4):444-454. http://www.ncbi.nlm.nih.gov/pubmed/33224595%0A http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7675163

Zhong M, Tian X, Sun Q, Li L, Lu Y, Feng Z, et al. Correlation of asprosin and Nrg-4 with type 2 diabetes Mellitus Complicated with Coronary Heart Disease and the Diagnostic Value. BMC Endocr Disord. 2023;23(1):1-8. https://doi.org/10.1186/s12902-023-01311-8

Deng X, Zhao L, Guo C, Yang L, Wang D, Li Y, et al. Higher serum asprosin level is associated with urinary albumin excretion and renal function in type 2 diabetes. Diabetes, Metab Syndr Obes. 2020;13:4341-4351. https://doi.org/10.2147/DMSO.S283413

Hameed MS, AL-Khakani MF. The Dual Role of Adiponectin and Leptin in Type 2 Diabetes. ‎Journal of Population Therapeutics and Clinical Pharmacology. 2023 Jun 3;30(13):200-14. ‎https://doi.org/10.47750/jptcp.2023.30.13.021

Cummings BP. Leptin therapy in type 2 diabetes. Diabetes, Obesity and Metabolism. 2013 Jul;15(7):607-12. https://doi.org/10.1111/dom.12048

Farrag M, Ait Eldjoudi D, González-Rodríguez M, Cordero-Barreal A, Ruiz-Fernández C, ‎Capuozzo M, ‎et al. Asprosin in health ‎and disease, a new glucose sensor ‎with central and peripheral metabolic effects. Frontiers in ‎Endocrinology. 2023 Jan 5;13:1101091. ‎https://doi.org/10.3389/fendo.2022.1101091

Naiemian S, Naeemipour M, Zarei M, Lari Najafi M, Gohari A, Behroozikhah MR, et al. Serum ‎concentration of asprosin in new-onset type 2 diabetes. Diabetology & Metabolic Syndrome. 2020 Dec;12:1-8.https://link.springer.com/content/pdf/10.1186/s13098-020-00564-w.pdf.

https://doi.org/10.1186/s13098-020-00564-w

Wang R, Hu W. Asprosin promotes β‐cell apoptosis by inhibiting the autophagy of β‐cell via AMPK‐mTOR ‎pathway. Journal of cellular physiology. 2021 Jan;236(1):215-21. https://doi.org/10.1002/jcp.29835

Romere C, Duerrschmid C, Bournat J, Constable P, Jain M, Xia F, et al. Asprosin, a fasting-induced glucogenic protein hormone. Cell. ‎‎2016 Apr 21;165(3):566-79.‎ https://doi.org/10.1016/j.cell.2016.02.063

Suryawanshi NP, Bhutey AK, Nagdeote AN, Jadhav AA, Manoorkar GS. Study of lipid peroxide and lipid ‎profile in diabetes mellitus. Indian journal of clinical Biochemistry. 2006 Mar;21:126-30.‎ https://link.springer.com/article/10.1007/BF02913080

https://doi.org/10.1007/BF02913080

Mathur S, Mehta DK, Kapoor S, Yadav S. Liver function in type-2 diabetes mellitus patients. Int J Sci Stud. 2016 Jan 1;3(10):43-7. https://d1wqtxts1xzle7.cloudfront.net/90922073/ijss_jan_oa09-libre.pdf?1662945424=&response-content-disposition=inline%3B+filename%3DLiver_Function_in_Type_2_Diabetes_Mellit