Evaluation of the protective effect of Omega-7 against Methotrexate Genotoxicity in bone marrow Cells of Mice

Zahraa Hasani; Ali F. Hassan

doi:10.32007/jfacmedbagdad.2116

Authors

Zahraa Hasani Ministry of Health and Environment https://orcid.org/0009-0006-5136-1617
Ali F. Hassan *Dept. Pharmacology and Toxicology College of Pharmacy, Universities of Baghdad.

DOI:

https://doi.org/10.32007/jfacmedbagdad.2116

Keywords:

Methotrexate, Omega-7, Chromosomal aberration, micronucleus appearance

Abstract

Background: A substance that can affect DNA or chromosomes is defined as a genotoxin. DNA damage in a somatic cell may result in a somatic mutation (cancer). In contrast, damage to a germ cell (germline mutation) may result in a heritable changed characteristic.Omega-7 is a non-essential monounsaturated free fatty acid with anti-inflammatory, anti-obesity, and antidiabetic effects.

Objectives: Evaluation of the possible protective effects of omega seven against methotrexate genotoxicity.

Method: Two major equal groups were obtained from seventy mice, and five subgroups (each of seven) were created from these groups as follows: Group I received liquid paraffin orally for seven successive days. Group II: received liquid paraffin orally for seven successive days, followed by a single intraperitoneal dose of methotrexate (20 mg/kg) on the eighth day. Group III: received omega-7 (50mg/kg) orally for seven successive days, followed by a single intraperitoneal dose of methotrexate (20 mg/kg) on the eighth day. Group IV: received omega-7 (100mg/kg) orally for seven successive days, followed by a single intraperitoneal dose of methotrexate (20 mg/kg) on the eighth day. Group V: received omega-7 (100mg/kg) orally for seven successive days. The first major group was intraperitoneally injected with 1mg/kg colchicine, and then after two hours, all mice were killed by spinal dislocation. Bone marrow cells from the first major group were used to measure the mitotic index and chromosomal aberrations, and bone marrow cell of the second group was used to measure the appearance of the micronucleus. Statistical Package for Social Sciences (SPSS) and ANOVA test were used to compare groups.

Results: Treatment of mice with omega-7 led to a significant decline in chromosomal aberration and micronucleus aberrance with a significant elevation of the mitotic index.

Conclusion: Omega-7 has been shown to have a protective role against methotrexate genotoxicity.

Received: April, 2023

Accepted: July, 2023

Published: Jan.2024

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References

Cunningham E. What are n-7 fatty acids and are there health benefits associated with them? J Acad Nutr Diet. 2015;115(2):324. DOI: 10.1016/j.jand.2014.11.021.https://doi.org/10.1016/j.jand.2014.11.021

García VL. The omega 7 as a health strategy for the skin and mucous membranes. EC Nutr. 2019; 14: 484–9. chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj /http://greenwood.ru/wp-content/uploads/2020/08/1.-The-Omega-7-as-a-Health-Strategy-for-the-Skin-and-Mucous-Membranes.pdf .

Hameed HA, Hassan AF. The Prophylactic Anti-Inflammatory Effect of Omega-7 Against Paracetamol-Induced Liver Injury in Rats. Iraqi J Vet Med. 2022;46(2):43–7. DOI: https://doi.org/10.30539/ijvm.v46i2.1412

Duckett SK, Volpi-Lagreca G, Alende M, Long NM. Palmitoleic acid reduces intramuscular lipid and restores insulin sensitivity in obese sheep. Diabetes, Metab Syndr Obes targets Ther. 2014;7:553. DOI https://doi.org/10.2147/DMSO.S72695 .

Singh B. Indian sea buckthorn. New Age Herbals Resour Qual Pharmacogn. 2018;29–54 . doi.org/10.1007/978-981-10-8291-7_3 .

Hu W, Fitzgerald M, Topp B, Alam M, O’Hare TJ. A review of biological functions, health benefits, and possible de novo biosynthetic pathway of palmitoleic acid in macadamia nuts. J Funct Foods. 2019;62:103520. doi.org/10.1016/j.jff.2019.103520.

Weimann E, Silva MBB, Murata GM, Bortolon JR, Dermargos A, Curi R, et al. Topical anti-inflammatory activity of palmitoleic acid improves wound healing. PLoS One. 2018; 13(10):e0205338. https://doi.org/10.1371/journal.pone.0205338

Ling C, An H, Li L, Wang J, Lu T, Wang H, et al. Genotoxicity evaluation of titanium dioxide nanoparticles in vitro: a systematic review of the literature and meta-analysis. Biol Trace Elem Res. 2021; 199:2057-76.doi.org/10.1007/s12011-020-02311-8 .

https://doi.org/10.1007/s12011-020-02311-8

Savale SK. Genotoxicity of drugs: Introduction, prediction and evaluation. Asian J Biomater Res. 2018; 4(6):1-29. https://www.researchgate.net/publication/334389865_Genotoxicity_of_Drugs_Introduction_Prediction_and_Evaluation

Kahtan M, Hassan AF, Al-Shawi NN, Numan IT, Bahader GA. The Genoprotective Activity of Aqueous Green Tea extract against Metronidazole and Tinidazole Genotoxic Effect. Res J Pharm Technol. 2020;13(2):889-94. https://doi.org/10.5958/0974-360X.2020.00168.7

Pacchierotti F, Stocchi V. Analysis of chromosome aberrations in somatic and germ cells of the mouse. In: Genotoxicity Assessment. Springer; 2013. p. 147–63. Pacchierotti F, Stocchi V. Analysis of chromosome aberrations in somatic and germ cells of the mouse. In: Genotoxicity Assessment. Springer; 2013. p. 147-63. https://doi.org/10.1007/978-1-62703-529-3_7

El–Alfy NZI, Mahmoud MF, El-Ashry SRG, Alqosaibi AI. Genotoxic effect of methotrexate on bone marrow chromosomes and DNA of male albino mice (Mus musculus). Egypt J Hosp Med. 2016; 64(1): 350–63 https://doi.org/10.12816/0029027.

Datta S, Singh J, Singh J, Singh S, Singh S. Assessment of genotoxic effects of pesticide and vermicompost treated soil with Allium cepa test. Sustain Environ Res. 2018; 28(4):171-8.

https://doi.org/10.1016/j.serj.2018.01.005

Bentli R, Parlakpinar H, Polat A, Samdanci E, Sarihan ME, Sagir M. Molsidomine prevents cisplatin-induced hepatotoxicity. Arch Med Res. 2013; 44(7):521-8. https://doi.org/10.1016/j.arcmed.2013.09.013

Kim YG, Baltabekova AZ, Zhiyenbay EE, Aksambayeva AS, Shagyrova ZS, Khannanov R, et al. Recombinant Vaccinia virus-coded interferon inhibitor B18R: Expression, refolding and a use in a mammalian expression system with a RNA-vector. PLoS One. 2017; 12 (12):e0189308 . https://doi.org/10.1002/jor.22565 .

Raghu Nadhanan R, Fan C, Su Y, Howe PRC, Xian CJ. Fish oil in comparison to folinic acid for protection against adverse effects of methotrexate chemotherapy on bone. J Orthop Res. 2014; 32(4):587–96. https://doi.org/10.1002/jor.22565 .

Hussein A, Ali SM. A short-term comparison between the effect of two different concentrations of methotrexate on ovarian tissues and function of female albino rats. J Fac Med Baghdad. 2022;64(4):280–5. https://doi.org/10.32007/jfacmedbagdad.6441967 .

Mahmood SH, Hassan AF. The Anti-Inflammatory Effect of Omega-7 Against Cisplatin in Rat Model. Iraqi J Vet Med. 2022;46(2):48–52. https://doi.org/10.30539/ijvm.v46i2.1413 .

Oufi HG. The cytogenetic effects of silibinin alone and in combination with methotrexate in mouse bone marrow. Eur J Pharmacol. 2018; 824:179–84. https://doi.org/10.1016/j.ejphar.2018.02.018.

Ito H, Kasama K, Naruse S, Shimura K. Antitumor effect of palmitoleic acid on Ehrlich ascites tumor. Cancer Lett. 1982;17(2):197–203. https://doi.org/10.1016/0304-3835(82)90032-5 .

Chakraborty T, Roy S, Barman M, Ray S. Cell cycle delay and colchicine like metaphase inducing effects of Scutellaria discolor Colebr. herb aqueous extract in Allium cepa root apical meristem cells. Cytologia (Tokyo). 2021; 86(3):255–60. https://doi.org/10.1508/cytologia.86.255 .

Mohammed BI, Al-Shawi NN, Hasan AF. Protective Effects of Aqueous Cranberry Fruit Extract against Genotoxicity Induced by Cisplatin in Mice Bone Marrow Cells. International Journal of Drug Delivery Technology. 2023; 13(1):401-406. DOI: 10.25258/ijddt.13.1.64.

Hassan AF, Abbas RF, Al-Shawi NN, Numan IT. Evaluation of the Genoprotective and Cytoprotective Activity of Vitamin K-7 Against Doxorubicin in Bone Marrow Cells and Spleen Cells of Rats. Medico-legal Updat. 2020; 20(4):963. https://www.researchgate.net/publication/347273038_Evaluation_of_the_Genoprotective_and_Cytoprotective_Activity_of_Vitamin_K 7_Against_Doxorubicin_in_Bone_Marrow_Cells_and_Spleen_Cells_of_Rats.

Wagner ED, Plewa MJ. CHO cell cytotoxicity and genotoxicity analyses of disinfection by-products: an updated review. J Environ Sci. 2017; 58: 64–76. https://doi.org/10.1016/j.jes.2017.04.021 .

van den Boogaard WMC, Komninos DSJ, Vermeij WP. Chemotherapy Side-Effects: Not All DNA Damage Is Equal. Cancers (Basel). 2022; 14(3):627. https://doi.org/10.3390/cancers14030627.

Al-khateeb HM, Barraj AH, Kadhim ZA. Effect of methotrexate on mice embryo liver. Iraqi J Sci. 2014; 55(2):374–81. https://www.iasj.net/iasj/download/25e39b381a546e1c .

Prokopchuk TM, Kondratenko EI, Bataeva U V. Evaluation of the mutagenic and antimutagenic potentials of plant raw materials for functional and food purposes. In: IOP Conference Series: Earth and Environmental Science. IOP Publishing; 2021. p. 42029. DOI 10.1088/1755-1315/839/4/042029.

Deori M, Boruah DC, Devi D, Devi R. Antioxidant and antigenotoxic effects of pupae of the muga silkworm Antheraea assamensis. Food Biosci. 2014;5: 108–14. https://doi.org/10.1016/j.fbio.2013.12.001

Abd MR, Hassan AF. The Ameliorative Effect of Fimasartan against Methotrexate-Induced Nephrotoxicity in Rats. Iraqi J Pharm Sci (P-ISSN 1683-3597 E-ISSN 2521-3512). 2022; 31(1):87–94. https://doi.org/10.31351/vol31iss1pp87-94 .

Kalantar M, Shirali S, Hasanvand A, Valizadeh M, Tavakoli R, Asadi M, et al. Ameliorative effects of hydroalcoholic extract of lavandula officinalis l. on methotrexate-induced oxidative stress in rats. Pharm Sci. 2016; 23(1):18–26. https://eurekamag.com/research/070/944/070944649.php .

`Wang J, Iannarelli R, Pucciarelli S, Laudadio E, Galeazzi R, Giangrossi M, et al. Acetylshikonin isolated from Lithospermum erythrorhizon roots inhibits dihydrofolate reductase and hampers autochthonous mammary carcinogenesis in Δ16HER2 transgenic mice. Pharmacol Res. 2020;161:105123. https://doi.org/10.1016/j.phrs.2020.105123 .

Fenech M, Knasmueller S, Bolognesi C, Bonassi S, Holland N, Migliore L, et al. Molecular mechanisms by which in vivo exposure to exogenous chemical genotoxic agents can lead to micronucleus formation in lymphocytes in vivo and ex vivo in humans. Mutat Res Mutat Res. 2016; 770:12–25. https://doi.org/10.1016/j.mrrev.2016.04.008 .

Kumar S, Kiso A, Kithan NA. Chromosome Banding and Mechanism of Chromosome Aberrations. Cytogenet Mol Strateg Anal Hered Mater. 2021;45. http://dx.doi.org/10.5772/intechopen.96242 .

Grosen A, Kelsen J, Hvas CL, Bellaguarda E, Hanauer SB. The influence of methotrexate treatment on male fertility and pregnancy outcome after paternal exposure. Inflamm Bowel Dis. 2017;23(4):561–9. https://doi.org/10.1097/MIB.0000000000001064 .

Drishya S, Dhanisha SS, Guruvayoorappan C. Antioxidant‐rich fraction of Amomum subulatum fruits mitigates experimental methotrexate‐induced oxidative stress by regulating TNF‐α, IL‐1β, and IL‐6 proinflammatory cytokines. J Food Biochem. 2022;46(4):e13855. https://doi.org/10.1111/jfbc.13855 .

Wang F, Cao Y, Guo Y, Zhu Z, Zhang C. Evaluation of antioxidant and antibacterial activities of lipid extracts from Eustigmatos cf. polyphem (Eustigmatophyceae) and preliminary identification of bioactive compound. Algal Res. 2021;59:102446. https://doi.org/10.1016/j.algal.2021.102446 .

Hashem EZ, Khodadadi M, Asadi F, Koohi MK, Eslami M, Hasani-Dizaj S, et al. The Antioxidant Activity of Palmitoleic Acid on the Oxidative Stress Parameters of Palmitic Acid in Adult Rat Cardiomyocytes. Ann Mil Heal Sci Res. 2016;14(3). https://doi.org/10.5812/amh.11467 .