Association between Serum Insulin and Interleukin-6 levels with Breast Cancer in Post-Menopausal Iraqi Women
DOI:
https://doi.org/10.32007/jfacmedbagdad.2111Keywords:
Obesity, Breast Cancer, Postmenopausal Women, Insulin, Interleukin-6.Abstract
Background: Obesity rates are increasing day by day affecting all populations at different ages. The most prevalent kind of cancer among women worldwide is breast cancer, with increasing rates in the present time and in the future. Substantial connections between obesity and breast cancer are demonstrated. Elevated circulating levels of insulin and interleukin-6 have a substantial link to obesity and breast cancer development.
Objective: This study aimed to examine the serum levels of insulin and interleukin-6 in postmenopausal breast cancer patients and to study the connection between these biomarkers and breast cancer development.
Method: In this research, 120 postmenopausal women actively participated., 30 had a body mass index of >30kg/m2 diagnosed with breast cancer and considered as obese (group 1), 30 with a body mass index of <30kg/m2 with breast cancer and considered as not obese (group 2), 30 had a body mass index of >30 kg/m2 who were apparently healthy and were obese (group 3), and 30 participants with a body mass index of <30 kg/m2 , were apparently healthy and were not obese (group 4). Five millilitres of venous blood were provided by each individual. Insulin and interleukin-6 levels in the blood were measured using ELISA (enzyme-linked immunosorbent assay) kits.
Results: Serum insulin levels were higher in the groups of obese participants (groups 1 and 3) than the groups of non-obese participants (groups 2 and 4), serum levels of interleukin-6 were the lowest in the healthy control group without obesity (group 4). Substantial moderate correlation was found between body mass index and both insulin and interleukin-6. Postmenopausal breast cancer risk is increased with elevations of serum insulin and interleukin-6.
Conclusions: Elevated levels of serum interleukin-6 and insulin were linked to higher body mass index. In addition, in addition interleukin-6 elevated levels were linked to the cancer state of the non-obese patients. Risk of breast cancer increased with higher levels of circulating insulin and intereukin-6.
Received: Mar,, 2023
Accepted: May, 2023
Published: Jan.2023
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References
.World Obesity Day 2022 - Accelerating action to stop obesity [Internet]. Available from: https://www.who.int/news/item/04-03-2022-world-obesity-day-2022-accelerating-action-to-stop-esity
Arnold M, Morgan E, Rumgay H, Mafra A, Singh D, Laversanne M, et al. Current and future burden of breast cancer: Global statistics for 2020 and 2040. The Breast. 2022;66:15-23. https://doi.org/10.1016/j.breast.2022.08.010
Ali ZM, Ali SH, Mohsen FY. Assessment of Some Hematological parameters in Iraqi Women with Different Breast Cancer Stages. Iraqi J Pharm Sci (P-ISSN 1683-3597, E-ISSN 2521-3512). 2020;29(2):99-106. https://doi.org/10.31351/vol29iss2pp99-106
Renehan AG, Tyson M, Egger M, Heller RF, Zwahlen M. Body-mass index and incidence of cancer: a systematic review and meta-analysis of prospective observational studies. Lancet. 2008;371(9612):569-78. https://doi.org/10.1016/S0140-6736(08)60269-X
Atoum MF, Alparrey H. Association of Leptin Receptor Q223R Gene Polymorphism and Breast Cancer Patients: A Case Control Study. Asian Pacific J Cancer Prev. 2022;23(1):177-82. https://doi.org/10.31557/APJCP.2022.23.1.177
Andò S, Gelsomino L, Panza S, Giordano C, Bonofiglio D, Barone I, et al. Obesity, leptin and breast cancer: Epidemiological evidence and proposed mechanisms. Cancers (Basel). 2019;11(1):1-27. https://doi.org/10.3390/cancers11010062
Sánchez-Jiménez F, Pérez-Pérez A, De la Cruz-Merino L, Sánchez-Margalet V. Obesity and breast cancer: role of leptin. Front Oncol. 2019;9:596.
https://doi.org/10.3389/fonc.2019.00596
Turki SG, AL-Naqeeb AA, Muhammed ZJ, Ahmed WH. Evaluation of Serum Leptin, Interlukein-6 and some Biochemical Parameters in Iraq obese Adult Patients. Medico-legal Updat. 2020;20(3):997.
Chaloob AK. Correlation of interleukin 6 (IL-6) with estrogen and progesterone receptor expression in breast cancer patients. J Fac Med Baghdad. 2010;52(4):438-40.
https://doi.org/10.32007/jfacmedbagdad.524952
Salman AH, Iqbal MN, Bakir WA. The In Situ Expression of IL-6 and IL-1? in breast cancer patients. Iraqi J Pharm Sci (P-ISSN 1683-3597, E-ISSN 2521-3512). 2008;17(2):55-62.
https://doi.org/10.31351/vol17iss2pp55-62
Wang Y, Wang H, Hegde V, Dubuisson O, Gao Z, Dhurandhar N V, et al. Interplay of pro-and anti-inflammatory cytokines to determine lipid accretion in adipocytes. Int J Obes. 2013;37(11):1490-8. https://doi.org/10.1038/ijo.2013.9
Krogh-Madsen R, Plomgaard P, Keller P, Keller C, Pedersen BK. Insulin stimulates interleukin-6 and tumor necrosis factor-α gene expression in human subcutaneous adipose tissue. Am J Physiol Metab. 2004;286(2):E234-8.
https://doi.org/10.1152/ajpendo.00274.2003
Rose DP, Vona-Davis L. The cellular and molecular mechanisms by which insulin influences breast cancer risk and progression. Endocr Relat Cancer. 2012;19(6):R225-41.
https://doi.org/10.1530/ERC-12-0203
Gallagher EJ, Fierz Y, Vijayakumar A, Haddad N, Yakar S, LeRoith D. Inhibiting PI3K reduces mammary tumor growth and induces hyperglycemia in a mouse model of insulin resistance and hyperinsulinemia. Oncogene. 2012;31(27):3213-22.
https://doi.org/10.1038/onc.2011.495
Draznin B. Mechanism of the mitogenic influence of hyperinsulinemia. Diabetol Metab Syndr. 2011;3(1):1-3. https://doi.org/10.1186/1758-5996-3-10
Liu Y, Petreaca M, Martins‐Green M. Cell and molecular mechanisms of insulin‐induced angiogenesis. J Cell Mol Med. 2009;13(11‐12):4492-504.
https://doi.org/10.1111/j.1582-4934.2008.00555.x
Rensing KL, Houttuijn Bloemendaal FM, Weijers EM, Richel DJ, Büller HR, Koolwijk P, et al. Could recombinant insulin compounds contribute to adenocarcinoma progression by stimulating local angiogenesis? Diabetologia. 2010;53(5):966-70.
https://doi.org/10.1007/s00125-010-1687-y
Bergman RN, Ader M, Huecking K, Van Citters G. Accurate assessment of β-cell function: the hyperbolic correction. Diabetes. 2002;51(suppl_1):S212-20.
https://doi.org/10.2337/diabetes.51.2007.S212
Erion K, Corkey BE. β-Cell failure or β-cell abuse? Front Endocrinol (Lausanne). 2018;9:532. https://doi.org/10.3389/fendo.2018.00532
Jiang ZY, He Z, King BL, Kuroki T, Opland DM, Suzuma K, et al. Characterization of multiple signaling pathways of insulin in the regulation of vascular endothelial growth factor expression in vascular cells and angiogenesis. J Biol Chem. 2003;278(34):31964-71.
https://doi.org/10.1074/jbc.M303314200
Fain JN, Madan AK. Insulin enhances vascular endothelial growth factor, interleukin-8, and plasminogen activator inhibitor 1 but not interleukin-6 release by human adipocytes. Metabolism. 2005;54(2):220-6. https://doi.org/10.1016/j.metabol.2004.08.016
Sun W, Lu J, Wu S, Bi Y, Mu Y, Zhao J, et al. Association of insulin resistance with breast, ovarian, endometrial and cervical cancers in non-diabetic women. Am J Cancer Res. 2016;6(10):2334.
Grivennikov S, Karin M. Autocrine IL-6 signaling: a key event in tumorigenesis? Cancer Cell. 2008;13(1):7-9. https://doi.org/10.1016/j.ccr.2007.12.020
Galic S, Oakhill JS, Steinberg GR. Adipose tissue as an endocrine organ. Mol Cell Endocrinol. 2010;316(2):129-39. https://doi.org/10.1016/j.mce.2009.08.018
Jamel ZF, Mahdi DS, Alsaimary IE. Statistical Association Between Age Groups, Body Mass Index and Cancer Grade with Serological Concentrations of Immunological Biomarkers (Interleukin IL-1β, IL-6) among Females with Breast Cancer. IIB. 21(26.25):1-523.
Macciò A, Madeddu C, Gramignano G, Mulas C, Floris C, Massa D, et al. Correlation of body mass index and leptin with tumor size and stage of disease in hormone-dependent postmenopausal breast cancer: preliminary results and therapeutic implications. J Mol Med. 2010;88(7):677-86. https://doi.org/10.1007/s00109-010-0611-8
Abd MK, Alsamarai ATS, A-Qader AM. Evaluation the level of Interleukin-6 and total protein levels on women with breast cancer. In: AIP Conference Proceedings. AIP Publishing LLC; 2022. p. 20027. https://doi.org/10.1063/5.0094134
Sulaiman MM, Salih KN, Alazzawy MA. The Role of Proinfalmatory Cytokines (Interleukin-1 Beta and 6) in Pathogenesis of Breast Cancer. Indian J Public Health. 2019;10(11). https://doi.org/10.5958/0976-5506.2019.03924.X
Thwani AN Al. Serum level of Interleukin-6 in Breast Cancer Iraqi Women. Iraqi J Cancer Med Genet. 2012;5(1). https://doi.org/10.29409/ijcmg.v5i1.77
Weisberg SP, McCann D, Desai M, Rosenbaum M, Leibel RL, Ferrante AW. Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest. 2003;112(12):1796-808. https://doi.org/10.1172/JCI200319246
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