Detection of BRAFV600E Biomarker In Patients With Colorectal Cancer Using Immunohistochemical Techniques / Clinico-Pathological Study

Authors

  • Dr.seror Alrubaiaee Alyarmouk Trainiong Center
  • Dr. Thaeer Al-taee Alyarmouk Training Center

DOI:

https://doi.org/10.32007/jfacmedbagdad.6411888

Keywords:

Keyword: CRC : colorectal cancer BRAF : v-raf murine sarcoma viral oncogene homolog B1

Abstract

Background :

Colorectal cancer (CRC) is the 4th common gastrointestinal cancer. (BRAFV600E) is a member of RAF family of serine/threonine protein kinases that function to regulate the (MAPK) / (ERK) pathway. BRAFV600E mutated CRC are associated with right-sided primary tumors, older women and high-grade tumors.

Aims of the study:

Study the immunohistochemical expression of BRAFV600E biomarker in a sample of Iraqi patients with colorectal cancer and the correlation of BRAFV600E expression with other clinicopathological variables such as patient's age and tumor grade.

 

Materials and Methods :

in this prospective study a total of 90 colorectal cases of a sample of Iraqi patients were collected from teaching labs of AL-Yarmouk Teaching Hospital including 60 cases of colorectal carcinoma and 30 cases of colorectal adenoma.

 

Result: BRAFV600E biomarker was positive in 16.6% of colorectal carcinoma group and negative in colorectal adenoma group.

 

Conclusions:

BRAF expression was higher in older patients ages & higher tumor stages and there is no expression of BRAFV600E in adenoma cases.

 

 

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Author Biographies

Dr.seror Alrubaiaee, Alyarmouk Trainiong Center

  • Seror Abdul Kareem Turkan AL-Rubaiaee : M.B.Ch.B, FIBMS (Path) Histopathologist at Alyarmouk Training Center

Dr. Thaeer Al-taee, Alyarmouk Training Center

  • Thaeer Jawad Kadhim Al-taee : Consultant Histopathologist, MBChB, FIBMS (Path) at Alyarmouk Training Center

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Published

2022-04-24

How to Cite

1.
Alrubaiaee SAKT, Jawad Kadhim Al-taee T. Detection of BRAFV600E Biomarker In Patients With Colorectal Cancer Using Immunohistochemical Techniques / Clinico-Pathological Study. JFacMedBagdad [Internet]. 2022 Apr. 24 [cited 2022 May 27];64(1):47-51. Available from: https://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1888