A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice
DOI:
https://doi.org/10.32007/jfacmedbagdad.5121217Keywords:
DNA, Eukaryotes, extraction, Mice, bone marrow, Sperm, Hind III digestion.Abstract
Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c).
Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodium Dodecyl Sulfate(SDS), and urea without using Proteinase-K digestion or ultracentrifugation.
Results: This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion.
Conclusion: Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications.
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