The Impact of Selenium and Levothyroxine on the Immune System of Hypothyroid Rats
DOI:
https://doi.org/10.32007/jfacmedbagdad.6612186الكلمات المفتاحية:
Hypothyroidism، IGg-IL6، Immune system، Levothyroxine، SC-SeNPsالملخص
Background: The immune system and thyroid hormones have an inverse relationship, but both thyroid hormones and selenium play a crucial role in the immune system. However, when the levels of thyroid hormones decrease (known as hypothyroidism), their effect on immune system cells and their relationship with selenium supplements in the body are not well understood.
Objectives: The aim of this study was to investigate the impact of Se-nanoparticles on thyroid hormones and the blastogenic response of lymphocytes in various lymphoid tissues in rats with hypothyroidism.
Methods: A group of female Wister rats aged three to four months were randomly placed into five groups (each group has 6 rats) and were given a basal diet on a daily basis. However, the daily water supply given to each group was different. The first group was the control group and received normal water, while the other four groups were given 0.02% methimazole in their drinking water every day for four weeks to induce hypothyroidism. Once the hypothyroidism was confirmed, the 3rd, 4th, and 5th experimental groups were given one of the following three treatments for four weeks, respectively: SC-SeNPs (0.1mg selenium per kg per day) as T1, Levothyroxine (0.9μg per 100g per day) as T2, or SC-SeNPs (0.1 mg selenium per kg per day) and Levothyroxine (0.9μg per 100g per day) as T3. In the end, blood samples were collected from euthanized animals using a high dose of anesthesia, and lymphoid tissue samples (spleen, Peyer’s patches and mesenteric lymph node) were preserved in 10% formalin.
Results: Our research has revealed that IgG, a humoral immunity marker, was found to be significantly decreased in hypothyroidism. However, when treated with levothyroxine alone or in combination with Sc-SeNPs, the level of cellular immunity marker, IL-6, was found to be significantly increased in hypothyroidism when compared to the control group. Additionally, we observed histopathological changes in the lymphoid tissue of hypothyroidism rats, which included depletion of the white pulp of spleen with congestion in the blood vessels, a decrease in the lymphoid follicle of Peyer’s patches, and a decrease in the primary and secondary follicles of the mesenteric lymph nodes .
Conclusion: Thyroid hormones play a role in regulating innate and adaptive immunity. The combination of selenium with levothyroxine is the most effective treatment for hypothyroidism and improving the activity of the immune system cells .
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Voorbij AM, Leegwater PA, Buijtels JJ, Daminet S, Kooistra HS. Central hypothyroidism in miniature Schnauzers. Journal of veterinary internal medicine. 2016;30(1):85-91. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4913645/.
Gurda BL, Bradbury AM, Vite CH. Canine and feline models of human genetic diseases and their contributions to advancing clinical therapies. The Yale Journal of Biology and Medicine. 2017;90(3):417. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612185/.
Allawi AA. Prevalence of Hypothyroidism in Chronic Kidney Disease among Sample of Iraqi Patients. Journal of the Faculty of Medicine Baghdad. 2013;55(2):97-101. https://doi.org/10.32007/jfacmedbagdad.552631.
Feldman EC, Fracassi F, Peterson ME. Feline Endocrinology: Editorial Edra; 2019. 704 p. https://cir.nii.ac.jp/crid/1130850071580497163
Van Poucke M, Van Renterghem E, Peterson ME, van den Berg MF, Stock E, Peelman LJ, et al. Association of recessive c. 430G> A (p.(Gly144Arg)) thyroid peroxidase variant with primary congenital hypothyroidism in cats. Journal of Veterinary Internal Medicine. 2022;36(5):1597-606. https://doi.org/10.1111/jvim.16524.
Al-Qayim M. Effects of Lactobacillus acidophilus on Pituitary-thyroid axis in growing rat. Adv Anim Vet Sci. 2015;3(5):269-75. http://dx.doi.org/10.14737/journal.aavs/2015/3.5.269.275.
De Leo S, Lee SY, Braverman LE. Hyperthyroidism. Lancet. 2016;388(10047):906-18. https://doi.org/10.1016/S0140-6736(16)00278-6.
Weheeb MA, Sultan KM, Al.Obaidy MW. Spiro metric Tests and Thyroid Hormone Concentrations in sample of Iraqi Patients with Chronic Obstructive Pulmonary Disease. Journal of the Faculty of Medicine Baghdad. 2014;56(3):278-82. https://doi.org/10.32007/jfacmedbagdad.563497.
de Oliveira JTS, Wenceslau AA, da Silva EB, de Lavor MSL, Guedes PEB, Carlos RSA. Canine Hypothyroidism with Neurological Disorders. Acta Scientiae Veterinariae. 2022;50(1):808. https://www.ufrgs.br/actavet/50-suple-1/CR_808.pdf.
Al-Zyadi AJH. The therapeutic role of alcoholic extract of fenugreek seeds on hypothyroidism state induced by thiourea and some blood parameters in adult male rabbits: Atyaf J. H. Al-Zyadi and Jawad K. Arrak. The Iraqi Journal of Veterinary Medicine. 2015;39(1):1-7. https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/187.
Jara EL, Muñoz-Durango N, Llanos C, Fardella C, González PA, Bueno SM, et al. Modulating the function of the immune system by thyroid hormones and thyrotropin. Immunology Letters. 2017;184:76-83. https://doi.org/10.1016/j.imlet.2017.02.010.
Sharma R. Hypothyroidism After Use of Immune Checkpoint Inhibitor Therapy in Patient With Graves' Disease: Cure? JCEM Case Reports. 2023;1(1):1-3. https://doi.org/10.1210/jcemcr/luac024.
Aqeeli MJS, Hassan HS. Evaluation of Hypothyroidism Patients’ Compliance to Thyroxine Drug in Baghdad City. Teikyo Medical Journal. 2022;45(2):5833-9. https://www.teikyomedicaljournal.com/article/evaluation-of-hypothyroidism-patients-compliance-to-thyroxine-drug-in-baghdad-city.
Sabea AM, Al-Qaiym MA. Biogenic Selenium Nanoparticles Synergizes Levothyroxine in the Treatment of Hypothyroidism at the Level of Genes Expression. European Chemical Buletin. 2023;12(1):16. https://www.eurchembull.com/uploads/paper/46e5b95eb1d345ea98359d1cc7af6327.pdf.
Wu Q, Rayman MP, Lv H, Schomburg L, Cui B, Gao C, et al. Low population selenium status is associated with increased prevalence of thyroid disease. The Journal of Clinical Endocrinology & Metabolism. 2015;100(11):4037-47. https://pubmed.ncbi.nlm.nih.gov/26305620/.
Wali AT, Al-Qayim MAJ. Biosynthesis, Characterization and Bioactivity of Selenium Nanoparticles Synthesized by Propolis. The Iraqi Journal of Veterinary Medicine. 2019;43(1):197-209. https://doi.org/10.30539/iraqijvm.v43i1.490.
Haddad, et al. Synthesis and Characterization of New Selenonitrone Derivative and Its Effect on Breast Cancer Cell Line Viability in Vitro. Baghdad Science Journal. 2019;16(3(Suppl.)):0754. https://doi.org/10.21123/bsj.2019.16.3(Suppl.).0754.
Ayad ZM, Ibrahim OMS, Omar LW. Biosynthesis and characterization of silver nanoparticles by Silybum marianum (silymarin) fruit extract. Adv Anim Vet Sci. 2019;7(2):122-30. https://nexusacademicpublishers.com/uploads/files/AAVS_7_2_122-130.pdf
Ghazi MA, Al-Qaiym MA. Role of T. vulgaris in protection DNA damage induced by Lead acetate in Rats. Journal of Biotechology Research Center. 2023;17(3):11. https://doi.org/10.24126/jobrc.2023.17.1.701.
De Luca R, Davis PJ, Lin H-Y, Gionfra F, Percario ZA, Affabris E, et al. Thyroid hormones interaction with immune response, inflammation and non-thyroidal illness syndrome. Frontiers in cell and developmental biology. 2021;8:614030. https://doi.org/10.3389/fcell.2020.614030.
Montesinos MdM, Pellizas CG. Thyroid hormone action on innate immunity. Frontiers in Endocrinology. 2019;10:350. https://doi.org/10.3389/fendo.2019.00350
Ibraheem SR, Mutashar EK, Salih AM. Detection of some Physiological and Immunological parameters in Iraqi clinical and subclinical hypothyroid patients: Relationship study. Current Research in Microbiology and Biotechnology. 2018;6(1):1411-9. Current Research in Microbiology and Biotechnology (researchgate.net)
Grondman I, de Nooijer AH, Antonakos N, Janssen NA, Mouktaroudi M, Leventogiannis K, et al. The association of TSH and thyroid hormones with lymphopenia in bacterial sepsis and COVID-19. The Journal of Clinical Endocrinology & Metabolism. 2021;106(7):1994-2009. https://pubmed.ncbi.nlm.nih.gov/33713408/.
Valli E, Dalotto‐Moreno T, Sterle HA, Méndez‐Huergo SP, Paulazo MA, García SI, et al. Hypothyroidism‐associated immunosuppression involves induction of galectin‐1‐producing regulatory T cells. The FASEB Journal. 2023;37(4):e22865. https://pubmed.ncbi.nlm.nih.gov/36934391/.
Torimoto K, Okada Y, Nakayamada S, Kubo S, Kurozumi A, Narisawa M, et al. Comprehensive immunophenotypic analysis reveals the pathological involvement of Th17 cells in Graves' disease. Scientific Reports. 2022;12(1):16880. https://pubmed.ncbi.nlm.nih.gov/36207336/
Hansen M, Johnson A, Weber KS, O’Neill KL. Characterizing the Interplay of Lymphocytes in Graves’ Disease. International Journal of Molecular Sciences. 2023;24(7):6835. https://doi.org/10.3390/ijms24076835.
Teke Kisa P, Arslan N. Inborn errors of immunity and metabolic disorders: current understanding, diagnosis, and treatment approaches. Journal of Pediatric Endocrinology and Metabolism. 2021;34(3):277-94. https://pubmed.ncbi.nlm.nih.gov/33675210/.
Shaji B, Joel JJ. Impact of Hypothyroidism on Metabolic and Cognitive Dysfunction: A Comprehensive Review. Journal of Young Pharmacists. 2022;14(4). https://jyoungpharm.org/5523/.
Chan AC, Carter PJ. Therapeutic antibodies for autoimmunity and inflammation. Nature Reviews Immunology. 2010;10(5):301-16. https://doi.org/10.1038/nri2761.
Li S, Sun W, Zhang K, Zhu J, Jia X, Guo X, et al. Selenium deficiency induces spleen pathological changes in pigs by decreasing selenoprotein expression, evoking oxidative stress, and activating inflammation and apoptosis. Journal of Animal Science and Biotechnology. 2021;12:1-13. https://doi.org/10.1186/s40104-021-00587-x.
Wu Z, Guo Z, Zheng Y, Wang Y, Zhang H, Pan H, et al. IgG N-Glycosylation Cardiovascular Age Tracks Cardiovascular Risk Beyond Calendar Age. Engineering. 2023. https://doi.org/10.1016/j.eng.2022.12.004.
Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease. Cold Spring Harbor perspectives in biology. 2014;6(10):a016295. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176007/.
Peixoto MS, e Souza AdV, Andrade IS, El Giusbi CdC, Faria CC, Hecht F, et al. Hypothyroidism induces oxidative stress and DNA damage in breast. Endocrine-Related Cancer. 2021;28(7):505-19. https://pubmed.ncbi.nlm.nih.gov/34010147/.
Wang Q, Huang J, Zheng Y, Guan X, Lai C, Gao H, et al. Selenium-enriched oolong tea (Camellia sinensis) extract exerts anti-inflammatory potential via targeting NF-κB and MAPK pathways in macrophages. Food Science and Human Wellness. 2022;11(3):635-42. https://doi.org/10.1016/j.fshw.2021.12.020.
Eid SY, El-Zaher HM, Emara SS, Farid OA-H, Michael MI. Nano selenium treatment effects on thyroid hormones, immunity and antioxidant status in rabbits. World Rabbit Science. 2019;27(2):93-100. https://doi.org/10.4995/wrs.2019.11251
Park S, Zhu X, Kim M, Zhao L, Cheng S-Y. Thyroid hormone receptor α1 mutants impair B lymphocyte development in a mouse model. Thyroid. 2021;31(6):994-1002. https://pubmed.ncbi.nlm.nih.gov/33267733/.
Wenzek C, Boelen A, Westendorf AM, Engel DR, Moeller LC, Führer D. The interplay of thyroid hormones and the immune system–where we stand and why we need to know about it. European Journal of Endocrinology. 2022;186(5):R65-R77. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010816/
Davis PJ, Glinsky GV, Lin H-Y, Leith JT, Hercbergs A, Tang H-Y, et al. Cancer cell gene expression modulated from plasma membrane integrin αvβ3 by thyroid hormone and nanoparticulate tetrac. Frontiers in Endocrinology. 2015;5:240. https://pubmed.ncbi.nlm.nih.gov/25628605/.
Rehman HFU, Zaneb H, Masood S, Yousaf MS, Ashraf S, Usman MM, et al. Effect of selenium nanoparticles and mannan oligosaccharide supplementation on muscle and lymphoid histomorphometry and morphometry of tibia bone in broilers reared under high stocking density. Turkish Journal of Veterinary & Animal Sciences. 2022;46(5):698-707. https://journals.tubitak.gov.tr/veterinary/vol46/iss5/4/.
Qing Z, Dongliu L, Xuedie G, Khoso PA, Xiaodan H, Shu L. MiR-144-3p targets STC1 to activate PI3K/AKT pathway to induce cell apoptosis and cell cycle arrest in selenium deficiency broilers. Journal of Inorganic Biochemistry. 2022;226:111665. https://doi.org/10.1016/j.jinorgbio.2021.111665.
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