Immunohistochemical Expression and Histopathological Role of CD47 in Colorectal Cancer in Iraqi Patients

Fatima O.  Abd alkareem; Ban J.  Mohamad

doi:10.32007/jfacmedbagdad.6622275

Authors

Fatima O. Abd alkareem Department of Biology, College of Science, University of Baghdad. Baghdad, Iraq. https://orcid.org/0009-0008-9712-3512
Ban J. Mohamad Department of Biology, College of Science, University of Baghdad. Baghdad, Iraq. https://orcid.org/0000-0003-3025-6632

DOI:

https://doi.org/10.32007/jfacmedbagdad.6622275

Keywords:

Colorectal cancer, Cluster of Differentiation 47, Immunohistochemistry, Integrin-associated protein.

Abstract

Background: Bowel cancer is the most prevalent digestive system cancer and is the 4^th largest cause of cancer-related death worldwide. In Iraq, colon and rectal cancer (CRC) is the 6^th most common malignancy in males and the 5^th in females. This cancer is sluggish in growth, which gives a window of opportunity to screen for both precursor lesions and early cancer. The Cluster of Differentiation 47 (CD47) protein is a type of transmembrane glycoproteins found on nearly all human cells, including non-hematopoietic and hematopoietic cells. CD47 promotes CRC growth by triggering angiogenesis and apoptosis of tumor cell.

Objectives: To evaluate the immunohistochemical expression of (CD47) in various colorectal samples from Iraqi patients with CRC by immunohistochemistry (IHC) assay.

Specimens and methods: A total of 45 paraffin-embedded CRC tissue specimens and clinical data were obtained from the Medical City Teaching Hospital in Baghdad and a number of private laboratories in Baghdad, Iraq. In addition, 30 control colon and rectum tissues with no significant pathology were collected from the Forensic Medicine Department for comparison purposes after taking the official approvals.

Results: The results revealed a high expression of CD47 in CRC cases, but with no significant correlation with clinicopathological features. Also, the result of figures in this study revealed negative membranous expression of CD47 (score 0), strong membranous expression of CD47 (score 3), moderately membranous expression of CD47 (score 2), and weak membranous expression of CD47 (score 1)

Conclusion: Patients with CRC had high CD47 expression, allowing tumor cells to modulate CD47SIRP inhibitory signaling and prevent immune cell attack.

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Author Biographies

Fatima O. Abd alkareem, Department of Biology, College of Science, University of Baghdad. Baghdad, Iraq.

Department of Biology, College of Science, University of Baghdad. Baghdad, Iraq
Ban J. Mohamad, Department of Biology, College of Science, University of Baghdad. Baghdad, Iraq.

Department of Biology, College of Science, University of Baghdad. Baghdad, Iraq.

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