Molecular detection of ALS1 virulence gene of Candida albicans isolated from groups of Iraqi patients

Aysha Saber Ali; Azhar A.F. Al-Attraqhchi; Saife D. Al-Ahmar

doi:10.32007/jfacmedbagdad.571317

Authors

Aysha Saber Ali Dept. of Microbiology, College of Medicine/Al-Nahrain University
Azhar A.F. Al-Attraqhchi Dept. of Microbiology, College of Medicine/Al-Nahrain University
Saife D. Al-Ahmar Genetic Engeneering Institute/University of Baghdad.

DOI:

https://doi.org/10.32007/jfacmedbagdad.571317

Keywords:

Candida albicans, ALS1 gene, PCR virulence gene.

Abstract

Background: Candida albicans is the principal fungal infectious agent in human infection. Adhesion is thought to be an essential step for colonization and establishment of Candida infections.
Objectives: Identification and comparison of ALS1 virulence gene of adhesion family among different isolates of Candida albicans by PCR.
Patients and methods: One hundred eight samples were collected from different group of Iraqi patients. All samples were culture on Sabouraud′s agar, CHROMagar for identification while API Candida kit confirmatory test and extracted DNA was done for just Candida albicans isolates, detected the ALS1 gene, extracted RNA for synthesis of cDNA and detected of gene and compare between isolates.
Result: C. albians isolated from each of vaginal and oral swabs expressed the stronger gene that mean these isolates were the most virulent once. The two isolates from each of urine sample and nail clip which expressed only one strong fluorescence represented by ALS1 gene. C. albicans isolated from cutaneous scrap sample showed the only one moderate fluorescence represented by ALS1 gene.
Conclusion: C. albians isolated from each of vaginal and oral swabs expressed the stronger gene that’s mean these isolates were the most virulent once.