Genotyping of HLA-class-I and II by PCR-SSP of Patients with Recurrent Aphthous Stomatitis

Batool H. Al-Ghurabei; Mustafa M. Saliah; Ahmed A . Al-Hassan; Ammra J. Al-Nema; Ahmed Kadem

doi:10.32007/jfacmedbagdad.541775

Authors

Batool H. Al-Ghurabei Clinical Immunology, College of Dentistry/ University of Baghdad
Mustafa M. Saliah College of Dentistry/ University of Baghdad.
Ahmed A . Al-Hassan Molecular Immunology, Medical College/ Al- Nahrain University
Ammra J. Al-Nema Clinical Biochemistry\HLA-Laboratory/Forencis Medicine Institute/Baghdad
Ahmed Kadem Genetics\HLA-Laboratory / Forencis Medicine Institute/Baghdad

DOI:

https://doi.org/10.32007/jfacmedbagdad.541775

Keywords:

RAS, HLA allele, PCR.

Abstract

Background: Recurrent aphthous stomatitis is a common condition characterised by recurrent episodes of oral ulceration. Genetic factors have been implicated by numerous studies on the association of recurrent aphthous stomatitis and the genetically determined HLA subtypes.
Objectives: Current study was established to shed light on the possible association of HLA class I and II alleles with recurrent aphthous stomatitis in Iraqi patients.
Subjects and Methods: The study included 55 subjects: 30 recurrent aphthous stomatitis patients and 25 apparently healthy subjects as control. Polymerase chain reaction-specific sequence primers (PCR- SSP) assay was conducted to assess HLA- typing.
Results: The present study showed a significant association of HLA-Cw*12:02:01-and HLADQB1* 02:01:01- alleles with recurrent aphthous stomatitis as compared with healthy control (P=
0.041and P=0.028 respectively). Another interesting finding in this study was the significant low frequency of HLA-DQB1*05:01:01- allele in patients when compared with healthy control (P=0.036).
Conclusion: These findings demonstrated that HLA-Cw*12:02:01- and HLA-DQB1*02:01:01-alleles may play a role in the etiology of the disease. Whereas HLA-DQB1*05:01:01-05 may confer protective effects against recurrent aphthous stomatitis.