A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice

  • Shayma J. Ahmed Department of Anatomy, college of Medicine, university of Baghdad
DOI: https://doi.org/10.32007/1217%25g220-222
Keywords: DNA, Eukaryotes, extraction, Mice, bone marrow, Sperm, Hind III digestion.

Abstract

Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c).
Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodium Dodecyl Sulfate(SDS), and urea without using Proteinase-K digestion or ultracentrifugation.
Results: This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion.
Conclusion: Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications.

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Published
2009-07-01
How to Cite
1.
Ahmed S. A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice. J Fac Med Bagdad [Internet]. 1Jul.2009 [cited 21Nov.2019];51(2):220-2. Available from: http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1217
Issue
Vol 51 No 2 (2009): Journal of the Faculty of Medicine Baghdad
Section
Articles

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